Evaluation of specific binding affinity and biochemical properties of fish eye-lens reagents from seven teleost species
1988; Elsevier BV; Volume: 90; Issue: 4 Linguagem: Inglês
10.1016/0305-0491(88)90326-4
ISSN0305-0491
AutoresB. L. Middlebrooks, Nancy J. Brown‐Peterson, Paul M. Toom, Willard L. Douglas,
Tópico(s)Venomous Animal Envenomation and Studies
Resumo1. Eye-lens proteins from seven teleost species were extracted and processed to produce modifications conferring specific binding affinity for selected molecules or cellular antigens. Specifically active reagents were obtained from two of the species utilized. 2. Eye-lens preparations contained several positively charged subunits, with the predominant subunit having a mol. wt of approx. 20,000. 3. Of a number of assay methods evaluated the passive hemagglutination test best assessed specific binding properties of eye-lens reagents. 4. The best specific binding activity was demonstrated for soluble protein molecules (e.g. rabbit IgG), with weak or no binding activity demonstrated for polysaccharide or cell-associated structures.
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