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Anti-inflammatory effects of IL-4 and IL-10 on Human Polymorphonuclear Leukocytes

2002; Korean Academy of Medical Sciences; Volume: 17; Issue: 1 Linguagem: Inglês

10.3346/jkms.2002.17.1.7

1598-6357

Sung Woo Lee, Yun Sik Hong, Chung Min Chun, Jun-Dong Moon, Su Jin Kim, In Chul Jung, Young‐Hoon Yoon, Be An Lee, Sung Woo Moon, Sung‐Hyuk Choi, Chul Kyu Moon,

Chemokine receptors and signaling

Inflammatory responses are strictly regulated by coordination of pro-inflammatory and anti-inflammatory mediators. Interleukin-4 (IL-4) and interleukin-10 (IL-10) have typically the biologic anti-inflammatory effects on monocytes, but uncertain effects on polymorphonuclear leukocytes (PMNs). The PMNs are the first line of cellular response for host defense during acute inflammation. To modify hyper-inflammatory reaction with biologic anti-inflammatory mediators, we have determined the biologic anti-inflammatory activities of IL-4 and IL-10 on human PMNs. Human PMNs were pretreated with IL-4 or IL-10 and then stimulated with formyl methionyl leucyl phenylalanine (fMLP) for times indicated. The level of H2O2, interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) were determined in the each cell free supernatants. fMLP plays the role of a typical pro-inflammatory agent and, at least in determined conditions, down-regulated TNF release. IL-4 acts as an anti-inflammatory mediator but IL-10 did not show its anti-inflammatory activities on fMLP-stimulated human PMNs. IL-4 and IL-10 have different anti-inflammatory mechanisms. Perhaps, IL-10 needs co-factors to act as an anti-inflammatory mediator.