Calnexin-dependent Enhancement of Nicotinic Acetylcholine Receptor Assembly and Surface Expression
1997; Elsevier BV; Volume: 272; Issue: 46 Linguagem: Inglês
10.1074/jbc.272.46.28925
ISSN1083-351X
AutoresWeise Chang, Marina S. Gelman, Joav Prives,
Tópico(s)Ion channel regulation and function
ResumoThe muscle-type nicotinic acetylcholine receptor (AChR)2 is a pentameric membrane ion channel assembled in the endoplasmic reticulum from four homologous subunits by mechanisms that are insufficiently understood. Nascent AChR subunits were recently found to form complexes with the endoplasmic reticulum-resident molecular chaperone calnexin. To determine the contribution of this interaction to AChR assembly and surface expression, we have now used transient transfection of mouse AChR subunits and calnexin into non-muscle cells. Co-transfection of calnexin along with AChR subunits into COS and HEK 293 cells was found to enhance AChR subunit folding and assembly, and to decrease degradation rates of newly synthesized AChR α-subunits, resulting in elevated surface expression of assembled AChR. Moreover, inhibition of the interaction between endogenous calnexin and AChR by castanospermine resulted in decreased AChR subunit folding, assembly, and surface expression in muscle and HEK 293 cells. Together, these findings provide evidence that calnexin directly contributes to AChR biogenesis by promoting subunit folding and assembly. The muscle-type nicotinic acetylcholine receptor (AChR)2 is a pentameric membrane ion channel assembled in the endoplasmic reticulum from four homologous subunits by mechanisms that are insufficiently understood. Nascent AChR subunits were recently found to form complexes with the endoplasmic reticulum-resident molecular chaperone calnexin. To determine the contribution of this interaction to AChR assembly and surface expression, we have now used transient transfection of mouse AChR subunits and calnexin into non-muscle cells. Co-transfection of calnexin along with AChR subunits into COS and HEK 293 cells was found to enhance AChR subunit folding and assembly, and to decrease degradation rates of newly synthesized AChR α-subunits, resulting in elevated surface expression of assembled AChR. Moreover, inhibition of the interaction between endogenous calnexin and AChR by castanospermine resulted in decreased AChR subunit folding, assembly, and surface expression in muscle and HEK 293 cells. Together, these findings provide evidence that calnexin directly contributes to AChR biogenesis by promoting subunit folding and assembly. Many of the transmembrane signaling molecules present in cell surface membranes are oligomeric proteins, and assembly of these proteins from constituent subunits is required for their functional expression. The nicotinic acetylcholine receptor (AChR), 1The abbreviations used are: AChR, acetylcholine receptor; ER, endoplasmic reticulum; CAS, castanospermine; Bgt, α-bungarotoxin; DMEM, Dulbecco's modified Eagle's medium; mAb, monoclonal antibody; MAP kinase, mitogen-activated protein kinase.1The abbreviations used are: AChR, acetylcholine receptor; ER, endoplasmic reticulum; CAS, castanospermine; Bgt, α-bungarotoxin; DMEM, Dulbecco's modified Eagle's medium; mAb, monoclonal antibody; MAP kinase, mitogen-activated protein kinase. a ligand-gated ion channel which mediates nerve to muscle transmission, is a pentamer which is assembled in the endoplasmic reticulum (ER) from four distinct subunits coded by different genes in the stoichiometry α2βγδ (1Merlie J.P. Cell. 1984; 36: 573-575Abstract Full Text PDF PubMed Scopus (34) Google Scholar, 2Galzi J.L. Revah F. Bessis A. Changeux J.P. Annu. Rev. Pharmacol. Toxicol. 1991; 31: 37-72Crossref PubMed Scopus (306) Google Scholar, 3Hall Z.W. Sanes J.R. Cell/Neuron. 1993; 71,10 (71,10, (suppl.) 99–121): 99-121Google Scholar, 4Karlin A. Akabas M.H. Neuron. 1995; 15: 1231-1244Abstract Full Text PDF PubMed Scopus (563) Google Scholar). The nascent AChR subunits are cotranslationally inserted into the ER membrane in a characteristic orientation with four transmembrane stretches, and with both the long amino-terminal domain and the short carboxyl terminus situated in the lumen of the ER. In the ER the newly translated subunits undergo post-translational modifications, including N-linked glycosylation and disulfide bond formation, as well as a sequence of folding events culminating in the acquisition of an assembly-competent conformation (5Merlie J.P. Smith M.M. J. Membr. Biol. 1986; 91: 1-10Crossref PubMed Scopus (40) Google Scholar). In the case of α-subunits, this conformational maturation results in the appearance of a specific epitope termed the main immunogenic region, which is recognized by conformation-specific antibodies to AChR, as well as the formation of high affinity binding sites for the neurotoxin α-bungarotoxin (Bgt) (1Merlie J.P. Cell. 1984; 36: 573-575Abstract Full Text PDF PubMed Scopus (34) Google Scholar, 6Conti-Tronconi B.M. McLane K.E. Raftery M.A. Grando S.A. Protti M.P. Crit. Rev. Biochem. Mol. Biol. 1994; 29: 69-123Crossref PubMed Scopus (143) Google Scholar). These sites appear on α-subunits prior to their assembly with other subunits: in contrast, the binding sites for AChR agonists such as acetylcholine and carbamylcholine arise only after the assembly of α- with γ- or δ-subunits (7Blount P. Merlie J.P. Neuron. 1989; 3: 349-357Abstract Full Text PDF PubMed Scopus (226) Google Scholar). Since in the assembled AChR these agonist sites partially overlap with the neurotoxin-binding sites (4Karlin A. Akabas M.H. Neuron. 1995; 15: 1231-1244Abstract Full Text PDF PubMed Scopus (563) Google Scholar), the fraction of Bgt binding that is preventable by the presence of carbamylcholine can serve as a measure of AChR assembly (8Kreienkamp H.-J. Maeda R.K. Sine S.M. Taylor P. Neuron. 1995; 14: 635-644Abstract Full Text PDF PubMed Scopus (92) Google Scholar). Only the assembled pentameric AChRs exit the ER and reach the cell surface, whereas unassembled or misfolded subunits and partial assembly intermediates are retained and degraded intracellularly (9Blount P. Smith M.M. Merlie J.P. J. Cell Biol. 1990; 111: 2601-2611Crossref PubMed Scopus (76) Google Scholar, 10Gu Y. Forsayeth J.R. Verrall S. Yu X.-M. Hall Z.W. J. Cell Biol. 1991; 114: 799-807Crossref PubMed Scopus (91) Google Scholar, 11Chavez R.A. Maloof J. Beeson D. Newsom-Davis J. Hall Z.W. J. Biol. Chem. 1992; 267: 23028-23034Abstract Full Text PDF PubMed Google Scholar), as is the case with other oligomeric proteins assembled in the ER (12Rose J.K. Doms R.W. Annu. Rev. Cell Biol. 1988; 4: 257-388Crossref PubMed Scopus (262) Google Scholar, 13Hurtley S.M. Helenius A. Annu. Rev. Cell Biol. 1989; 5: 277-307Crossref PubMed Scopus (776) Google Scholar). The expression of recombinant AChR subunits in heterologous cell lines has been used to address a number of fundamental questions relating to AChR assembly, such as the order in which the subunits are assembled (10Gu Y. Forsayeth J.R. Verrall S. Yu X.-M. Hall Z.W. J. Cell Biol. 1991; 114: 799-807Crossref PubMed Scopus (91) Google Scholar, 14Forsayeth J.R. Gu Y. Hall Z.W. J. Cell Biol. 1992; 117: 841-847Crossref PubMed Scopus (45) Google Scholar), the role of post-translational modifications in the regulation of AChR assembly (15Blount P. Merlie J.P. J. Cell Biol. 1990; 111: 2613-2622Crossref PubMed Scopus (93) Google Scholar, 16Sumikawa K. Gehle V.M. J. Biol. Chem. 1992; 267: 6286-6290Abstract Full Text PDF PubMed Google Scholar), and the identity of domains that form contacts between the subunits (8Kreienkamp H.-J. Maeda R.K. Sine S.M. Taylor P. Neuron. 1995; 14: 635-644Abstract Full Text PDF PubMed Scopus (92) Google Scholar). However, this approach is hampered by the low levels of correctly assembled AChR at the surface of transfected cells. This may reflect the tendency of nascent subunits to misfold and aggregate, a problem frequently encountered in the expression of recombinant proteins in various transfection systems. Under normal conditions, newly synthesized membrane proteins are thought to be protected from misfolding by interactions with ER-resident molecular chaperones (17Gething M.-J. Sambrook J. Nature. 1992; 355: 33-45Crossref PubMed Scopus (3591) Google Scholar, 18Gaut J.R. Hendershot L.M. Curr. Opin. Cell Biol. 1993; 5: 589-595Crossref PubMed Scopus (117) Google Scholar). Calnexin, an ER constituent that is itself a transmembrane protein (19Wada I. Rindress D. Cameron P.H. Ou W.-J. Doherty II, J.J. Louvard D. Bell A.W. Dignard D. Thomas D.Y. Bergeron J.J.M. J. Biol. Chem. 1991; 266: 19599-19610Abstract Full Text PDF PubMed Google Scholar, 20Hammond C. Helenius A. Curr. Biol. 1993; 3: 884-886Abstract Full Text PDF PubMed Scopus (46) Google Scholar, 21Bergeron J.J.M. Brenner M.B. Thomas D.Y. Williams D.B. Trends Biochem. Sci. 1994; 19: 124-128Abstract Full Text PDF PubMed Scopus (458) Google Scholar), has recently been shown to mediate folding and assembly of two oligomeric membrane glycoproteins: major histocompatibility complex class II molecules (22Vassilakos A. Cohen-Doyle M.F. Peterson P. Jackson M. Williams D. EMBO J. 1996; 15: 1495-1506Crossref PubMed Scopus (167) Google Scholar) and influenza hemagglutinin (23Hebert D.N. Foellmer B. Helenius A. EMBO J. 1996; 15: 2961-2968Crossref PubMed Scopus (255) Google Scholar). We have recently observed that calnexin forms transient complexes with newly synthesized AChR α-subunits in muscle cells, as well as in COS cells transiently transfected with this subunit (24Gelman M.S. Chang W. Thomas D.Y. Bergeron J.J.M. Prives J.M. J. Biol. Chem. 1995; 270: 15085-15092Abstract Full Text Full Text PDF PubMed Scopus (72) Google Scholar). Other transfected AChR subunits were also found to interact with calnexin in HEK 293 cells (25.Chang, W. (1995) Regulation of Acetylcholine Receptor: Phosphorylation, Assembly, and Clustering. Ph.D. thesis, State University of New York at Stony Brook, Stony Brook, NY.Google Scholar, 26Keller S.H. Lindstrom J. Taylor P. J. Biol. Chem. 1996; 271: 22871-22877Abstract Full Text Full Text PDF PubMed Scopus (56) Google Scholar). In muscle cells, the timing of this interaction appears to be precisely regulated, with binding occurring immediately upon subunit translation and dissociation taking place prior to AChR assembly (24Gelman M.S. Chang W. Thomas D.Y. Bergeron J.J.M. Prives J.M. J. Biol. Chem. 1995; 270: 15085-15092Abstract Full Text Full Text PDF PubMed Scopus (72) Google Scholar, 27Gelman M.S. Prives J.P. J. Biol. Chem. 1996; 271: 10709-10715Abstract Full Text Full Text PDF PubMed Scopus (21) Google Scholar). This time course is consistent with the possibility that calnexin assists AChR assembly by facilitating the folding of nascent subunits into an assembly-competent conformation. However, direct functional evidence that calnexin promotes maturation and assembly of AChR subunits in the ER has been lacking. In the present study we have examined the contribution of calnexin to AChR biogenesis in two ways: first, we took advantage of the inefficient folding and assembly of recombinant AChR subunits in transfected cells to determine if co-transfection of exogenous calnexin into these cells can increase AChR expression. Second, the ability of the glucose trimming inhibitor castanospermine (CAS) to block the interaction of endogenous calnexin with its substrates (28Hebert D.N. Foellmer B. Helenius A. Cell. 1995; 81: 425-433Abstract Full Text PDF PubMed Scopus (488) Google Scholar) was utilized to examine the effect of this block on AChR biogenesis. COS cells (African green monkey kidney cells) and HEK 293 cells (human embryonic kidney cells) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum. C2C12 mouse muscle cells were grown in DMEM supplemented with 10% fetal bovine serum, and induced to differentiate by replacing this medium with DMEM, 2% horse serum. Muscle primary cultures were prepared from breast muscle of 12-day-old chick embryos as described previously (29Ross A.F. Rapuano M. Schmidt J.H. Prives J.P. J. Biol. Chem. 1987; 262: 14640-14647Abstract Full Text PDF PubMed Google Scholar), plated on collagen-coated culture dishes at initial densities of 6 × 106 cells/100-mm culture dish, and grown in DMEM supplemented with 10% horse serum and 2% chick embryo extract. 35S-Protein labeling mixture (specific activity 1175 Ci/mmol) and 125I-α-bungarotoxin (specific activity 13–15 μCi/μg) were purchased from NEN Life Science Products (Boston, MA). Polyacrylamide gel electrophoresis reagents were from Bio-Rad. All other reagents were from Sigma. Anti-α-bungarotoxin antibody was raised in rabbits and affinity purified on α-bungarotoxin-Sepharose (29Ross A.F. Rapuano M. Schmidt J.H. Prives J.P. J. Biol. Chem. 1987; 262: 14640-14647Abstract Full Text PDF PubMed Google Scholar). The monoclonal antibody mAb 35, which recognizes the main immunogenic region of AChR α-subunit (30Tzartos S.J. Rand D.E. Einarson B.L. Lindstrom J.M. J. Biol. Chem. 1981; 256: 8635-8645Abstract Full Text PDF PubMed Google Scholar) was isolated from the supernatant of hybridoma TIB 175 (American Type Culture Collection). The monoclonal antibody mAb 61, directed against mouse AChR α-subunit was provided by Dr. Jon Lindstrom (University of Pennsylvania, Philadelphia, PA). Anti-chick AChR α-subunit antibody and anti-chick AChR δ-subunit antibody were raised in rabbits against the respective subunits purified on SDS-polyacrylamide gel electrophoresis from denervated chick leg muscle, and were shown to be noncross-reactive (29Ross A.F. Rapuano M. Schmidt J.H. Prives J.P. J. Biol. Chem. 1987; 262: 14640-14647Abstract Full Text PDF PubMed Google Scholar). Anti-calnexin antibody made against a synthetic peptide corresponding to a highly conserved stretch near the COOH terminus (amino acids 487–505) of calnexin was provided by Dr. J. J. M. Bergeron (McGill University, Montreal, Canada). Anti-HA 12CA5 antibody was obtained from Dr. D. Bar-Sagi (SUNY at Stony Brook, Stony Brook, NY). Full-length cDNAs coding for mouse AChR subunits (cDNA for α-subunit was obtained from Dr. Jim Boulter, Salk Institute, La Jolla, CA; cDNAs for β-, γ-, and δ-subunits were provided by Dr. James Patrick, Baylor College of Medicine, Houston, TX) were subcloned into the pRC/cytomegalovirus expression vector (Invitrogen, San Diego, CA), which contains an SV40 origin and is driven by the cytomegalovirus promoter. Calnexin cDNA was obtained from Dr. David Thomas (National Research Council of Canada, Montreal, Canada) and was subcloned into pBK/cytomegalovirus expression vector (Stratagene, La Jolla, CA). The HA Erk2 mitogen-activated protein kinase (MAP kinase) expression vector under the control of cytomegalovirus promoter was provided by Dr. D. Bar-Sagi (SUNY at Stony Brook, Stony Brook, NY). Transfection of COS and HEK 293 cells for transient expression of AChR was carried out by DNA-calcium phosphate precipitation as described (31Sambrook J. Fritsch E.E. Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New York1989Google Scholar). Briefly, 60-mm dishes of cells at 50–60% confluence were incubated for 16–20 h at 37 °C with 5–10 μg of the cDNA to be transfected in a mixture containing CaCl2 and HEPES-buffered saline solution. In cases when transfections were carried out with all four AChR subunits, the ratio of cDNAs encoding each subunit was α:β:γ:δ = 2:1:1:1. To rule out the possibility that any differences in transfection efficiencies contribute to the observed effects of co-transfected calnexin, the Escherichia coliβ-galactosidase was added to the transfection mixture and its expression was measured by colorimetric assay as described (31Sambrook J. Fritsch E.E. Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New York1989Google Scholar). Cultures were incubated in methionine-free DMEM for 1 h, then labeled at 37 °C with a mixture of [35S]methionine and [35S]cysteine (for specific activities see figure legends) in methionine-free DMEM. Chases were performed by washing cells once with DMEM, followed by incubation in DMEM supplemented with 5 mml-methionine. Incubations were terminated by two washes with ice-cold Dulbecco's phosphate-buffered saline, and subsequently cultures were harvested by scraping and extracted for 30 min at 4 °C in STE buffer (150 mm NaCl, 10 mm Tris-HCl, pH 7.4, 2 mm EGTA, 2 mm EDTA) containing 1 mg/ml aprotinin, 10 mm N-ethylmaleimide, 1 mm phenylmethylsulfonyl fluoride, and supplemented with 1% Triton X-100. Extracts were clarified by centrifugation for 15 min at 12,000 × gin a microcentrifuge at 4 °C. The clarified supernatants were incubated at 4 °C with the specified antiserum for 3 h, then with protein A-Sepharose beads (for anti-Bgt) or rabbit anti-rat antibody-coated protein A-Sepharose beads (for mAb 61, mAb 35) for a further 1 h. The precipitates were washed five times with STE-Triton X-100 and fractionated on 10% SDS-polyacrylamide gels. Radioactive bands were visualized by radiofluorography and quantified by densitometry or PhosphorImaging. COS cells were transfected with all four AChR subunits or with HA-epitope-tagged MAP kinase in the absence or presence of calnexin cDNA. After 2 days cells were scraped into STE, 1% Triton X-100 buffer at 4 °C, and extracts were clarified as above. Aliquots of lysates from calnexin co-transfected and control cultures, normalized for the transfection efficiency, were fractionated by SDS-polyacrylamide gel electrophoresis. Proteins were then transferred onto nitrocellulose membranes, which were incubated either with anti-calnexin or anti-HA antibody. The blots were processed using the enhanced chemiluminescence technique (Amersham). The amounts of cell surface and intracellular AChR were determined as described by Kreienkamp et al. (8Kreienkamp H.-J. Maeda R.K. Sine S.M. Taylor P. Neuron. 1995; 14: 635-644Abstract Full Text PDF PubMed Scopus (92) Google Scholar). For quantification of cell surface AChR, cultures were washed once with DMEM, then cells were detached in phosphate-buffered saline, 5 mm EDTA, resuspended in K+-Ringers buffer (140 mm KCl, 5.4 mm NaCl, 1.8 mmCaCl2, 1.7 mm MgCl2, 25 mm HEPES, pH 7.4, 0.03 mg/ml bovine serum albumin) and incubated with 10 nm125I-α-Bgt for 2 h at 4 °C. At the end of the incubation the cells were washed three times with K+-Ringer's buffer, and binding was quantified by γ-counting. For determination of nonspecific binding, the cells were preincubated with the AChR ligand carbamylcholine (10 mm) for 20 min prior to the addition of125I-Bgt, and labeling was carried out in the presence of carbamylcholine. To measure total folded α-subunit, labeling was carried out as above, except that cells were incubated with125I-Bgt in saponin permeabilization buffer (10 mm sodium phosphate, pH 7.5, 10 mm EDTA, 0.1% bovine serum albumin, 0.5% saponin). Total assembled AChR was estimated as the component of 125I-Bgt labeling that is prevented in the presence of 10 mm carbamylcholine. The unassembled folded AChR α-subunit was quantified as the component of125I-Bgt binding that is preserved in the presence of carbamylcholine, after subtraction of nonspecific binding estimated by the quantification of 125I-Bgt bound to nontransfected cultures. Fig.1 A compares AChR subunit synthesis, assembly, and surface expression in three cell types: C2C12 mouse muscle cells which normally express AChR upon differentiation, as well as COS and HEK 293 cells transiently transfected with the four (αβγδ) mouse AChR subunits. To measure AChR α-subunit synthesis, equivalent amounts of each of these cell types were labeled for 1 h with [35S]cysteine/methionine mixture, then extracted and immunoprecipitated with mAb 61, an antibody directed against mouse AChR α-subunit. This approach showed substantial levels of AChR α-subunit synthesized in muscle cells (lane 2), and even higher levels in transfected COS cells and HEK 293 cells (lanes 3 and 4). In parallel experiments, assembled and surface AChR were quantified in these metabolically labeled cells using the highly specific AChR ligand α-Bgt. To measure total assembled AChR, cells were permeabilized with saponin prior to incubation with Bgt (8Kreienkamp H.-J. Maeda R.K. Sine S.M. Taylor P. Neuron. 1995; 14: 635-644Abstract Full Text PDF PubMed Scopus (92) Google Scholar). Under these conditions Bgt can bind to both assembled AChR and unassembled but correctly folded α-subunits. To ascertain that the radiolabeled band in anti-Bgt immunoprecipitates shown in the middle panel of Fig. 1 corresponds predominantly to assembled α-subunit, the amounts of125I-Bgt binding in the presence and absence of 10 mm carbamylcholine were compared. Since the carbamylcholine-binding sites are comprised of domains from sets of 2 subunits (α-γ and α-δ), carbamylcholine will bind (and consequently block Bgt binding) to assembled α-subunit but not to unassembled α-subunit (8Kreienkamp H.-J. Maeda R.K. Sine S.M. Taylor P. Neuron. 1995; 14: 635-644Abstract Full Text PDF PubMed Scopus (92) Google Scholar). The contribution of these unassembled subunits to the total binding was not significant (<5%, data not shown). It should be noted that in contrast to the α-subunit which is clearly identifiable as a 40-kDa band, the other 3 subunits are difficult to resolve in [35S]methionine-labeled immunoprecipitates of assembled AChR, presumably due to their migration as diffuse bands, susceptibility to proteolysis, and the relatively high backgrounds in this region of the gel (15Blount P. Merlie J.P. J. Cell Biol. 1990; 111: 2613-2622Crossref PubMed Scopus (93) Google Scholar, 24Gelman M.S. Chang W. Thomas D.Y. Bergeron J.J.M. Prives J.M. J. Biol. Chem. 1995; 270: 15085-15092Abstract Full Text Full Text PDF PubMed Scopus (72) Google Scholar, 29Ross A.F. Rapuano M. Schmidt J.H. Prives J.P. J. Biol. Chem. 1987; 262: 14640-14647Abstract Full Text PDF PubMed Google Scholar). In replicate cultures, cell surface AChR was measured by the binding of Bgt to intact cells. After incubation with Bgt, cells were extracted and AChR-Bgt complexes were immunoprecipitated with anti-Bgt antibody. As can be seen in Fig. 1 A, in C2C12 muscle cells during a 3-h chase period a significant proportion of the labeled α-subunit was assembled into AChR pentamers (lane 5), and reached the cell surface (lane 8). In contrast, in each of the transfected cell lines during the same interval only a small fraction of the total α-subunit synthesized underwent assembly with other subunits (lanes 6 and 7) and appeared at the plasma membrane (lanes 9 and 10). These results are quantified in Fig. 1 B, which shows that the transfected HEK 293 and COS cells each display a substantially lower efficiency of AChR assembly and surface expression relative to the muscle cell line. To determine if the low levels of AChR assembly in transfected cells overexpressing the individual subunits reflect the exhaustion of endogenous calnexin, we measured the effect of co-transfecting calnexin cDNA on AChR assembly and surface expression. In the experiments shown in Fig.2 A, COS and HEK 293 cells were transfected with all four AChR subunits with or without calnexin, and 2 days later assembly was measured by the binding of 125I-Bgt to saponin-permeabilized cells, while surface AChR was quantified by125I-Bgt binding to intact cells. As can be seen, the levels of both assembled and surface AChR were markedly higher in cultures co-transfected with calnexin, consistent with the possibility that AChR assembly in the transfected cells was limited by the availability of this molecular chaperone. In contrast, co-transfection with calnexin did not cause the translocation of unassembled α-subunit to the plasma membrane, since no appreciable surface Bgt binding was detected when calnexin was co-transfected into either COS or HEK 293 cells expressing α-subunit alone (Fig. 2 A, first two bars in each panel). The overall levels of calnexin, as measured by Western blotting, were higher in calnexin-transfected cultures (Fig.2 C, top panel) (typically by 20–30%). Since the proportion of transfected cells in these experiments was between 5 and 10%, as determined by the β-galactosidase staining of fixed cultures (not shown), the levels of transfected calnexin in the transfected cells were estimated to significantly exceed (by 4–10-fold) those of endogenous calnexin. Together these results indicate that the increase in Bgt binding reflects specific stimulation of AChR biogenesis by co-transfected calnexin. The effects of calnexin co-transfection on AChR assembly and surface expression were also examined by a second method: immunoprecipitation of Bgt-AChR complexes from [35S]methionine/cysteine-labeled COS cells that had been transfected with all four AChR subunits with or without calnexin. As can be seen in Fig. 2 B, the amounts of both total (intracellular + surface) assembled AChR and surface AChR were increased in the cultures co-transfected with calnexin (lanes 4 and 6 versus lanes 3 and 5). Interestingly, the overall levels of radiolabeled AChR α-subunit accumulated during the 1-h labeling period were also higher in cells co-transfected with calnexin as compared with cultures transfected with AChR subunits but not with calnexin (Fig. 2 B, comparelanes 1 and 2). The increased accumulation of α-subunit in calnexin co-transfected cells was also evident from the Western blot (Fig. 2 C, middle panel). In contrast, the levels of a transfected cytoplasmic protein, MAP kinase, were not altered by co-transfection with calnexin (Fig. 2 C, bottom panel), consistent with the known localization of calnexin to the ER and the restriction of its chaperone functions to secretory and membrane proteins. It is noteworthy that the enhancement by co-transfected calnexin of AChR assembly and surface expression appeared to be greater than the increase in α-subunit levels in these cultures (Fig. 2 B). Thus, calnexin may contribute to AChR expression in two ways: by stabilizing newly synthesized AChR subunits in the ER, and by mediating the assembly of these subunits into pentameric AChR expressed on the cell surface. Calnexin has been proposed to mediate the folding of nascent AChR subunits prior to their assembly into multisubunit complexes based on the timing of its interaction with α-subunit in cultured muscle cells (24Gelman M.S. Chang W. Thomas D.Y. Bergeron J.J.M. Prives J.M. J. Biol. Chem. 1995; 270: 15085-15092Abstract Full Text Full Text PDF PubMed Scopus (72) Google Scholar). To directly investigate the contribution of calnexin to the conformational maturation of AChR α-subunit, we have measured the ability of conformationally mature α-subunits to bind Bgt. As can be seen in Fig. 3, calnexin co-transfection leads to an increase in intracellular125I-Bgt binding to α-subunit expressed both in COS and HEK 293 cells. Thus elevation of calnexin levels appears to facilitate the folding of nascent AChR α-subunits in the transfected cells. In the transfection experiments summarized above we have observed that calnexin has stimulatory effects on the folding and assembly of AChR α-subunit, as well as on the accumulation of newly made α-subunits in the ER. As net accumulation represents the difference between biosynthesis and degradation, we next determined the effect of co-transfected calnexin on the degradation rate of α-subunit. For this purpose, COS cells were transfected with all four AChR subunits in the absence or presence of calnexin, and after 2 days these cultures were pulse-labeled for 15 min with [35S]methionine/cysteine and chased for the specified periods in the absence of radioactive amino acids. At the end of the chase periods, cells were extracted and immunoprecipitated with mAb 61. The intensities of the bands corresponding to the radiolabeled α-subunit were quantified by scanning densitometry of autoradiographs. To compare α-subunit degradation rates in the absence and presence of co-transfected calnexin, the values at each time point were expressed as the proportion of the radiolabeled α-subunit immunoprecipitated immediately after the pulse. In a representative experiment shown in Fig.4, the disappearance of α-subunit in COS cells showed first-order kinetics with a half-time of approximately 1.5 h. This value is in good agreement with the catabolic half-life of mouse AChR α-subunit previously measured in COS cells (11Chavez R.A. Maloof J. Beeson D. Newsom-Davis J. Hall Z.W. J. Biol. Chem. 1992; 267: 23028-23034Abstract Full Text PDF PubMed Google Scholar) and in quail fibroblasts (15Blount P. Merlie J.P. J. Cell Biol. 1990; 111: 2613-2622Crossref PubMed Scopus (93) Google Scholar, 32Blount P. Merlie J.P. J. Biol. Chem. 1988; 263: 1072-1080Abstract Full Text PDF PubMed Google Scholar). In the presence of co-transfected calnexin α-subunit degradation rate was diminished significantly (half-time of approximately 4 h), indicating that interaction with calnexin can stabilize nascent AChR subunits in the ER. This decrease in AChR α-subunit degradation is likely to account for the enhanced accumulation of α-subunit observed in calnexin co-transfected cells (see Fig. 2 B, lanes 1 and 2; Fig. 2 C, middle panel). Moreover, co-transfected calnexin had a similar stabilizing effect on cultures transfected with α-subunit alone (not shown). In the experiments described thus far, co-transfection with calnexin was seen to enhance AChR biogenesis in cells that are inefficient in the assembly and surface expression of these receptors. We next examined the contribution of calnexin to AChR expression in primary cultures of embryonic chick myotubes, a cell system that assembles AChR with high efficiency (29Ross A.F. Rapuano M. Schmidt J.H. Prives J.P. J. Biol. Chem. 1987; 262: 14640-14647Abstract Full Text PDF PubMed Google Scholar). In these experiments we utilized the glucosidase inhibitor CAS, that was recently shown to interfere with the binding of calnexin to nascent glycoproteins (28Hebert D.N. Foellmer B. Helenius A. Cell. 1995; 81: 425-433Abstract Full Text PDF PubMed Scopus (488) Google Scholar). C
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