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Simultaneous determination of sucrose and reducing sugars using indirect flow-injection biamperometry

1993; Elsevier BV; Volume: 271; Issue: 2 Linguagem: Inglês

10.1016/0003-2670(93)80051-l

1873-4324

Jacek Michao̵wski, Anatol Kojo̵, Marek Trojanowicz, Bogdan Szostek, Elias A.G. Zagatto,

Analytical Chemistry and Sensors

The method developed for the determination of sugars is based on reduction hexacyanoferrate(III) by simple carbohydrates in a strongly alkaline medium at elevated temperature. Changes in hexacyanoferrate (III) concentration are monitored amperometrically with platinum wire electrodes polarized at 200 mV. Sucrose determination requires on-line hydrolysis in hydrochloric acid. The detection sensitivity for different simple sugars and disaccharides and the influence of several interferents were examined. Sucrose and glucose were determined with a sampling rate of 40 h−1 in natural samples from a sugar production process spiked with glucose.