Produção Nacional
Revisado por pares
E ‐cadherin downregulation and T wist overexpression since early stages of oral carcinogenesis
2013; Wiley; Volume: 43; Issue: 2 Linguagem: Inglês
10.1111/jop.12096
ISSN1600-0714
AutoresBrunno Santos de Freitas Silva, Fernanda Paula Yamamoto‐Silva, Hélder Antônio Rebelo Pontes, Décio dos Santos Pinto Júnior,
Tópico(s)Hedgehog Signaling Pathway Studies
ResumoThere is some evidence of Twist participation in oral carcinogenesis; however, little is known about its interaction with E-cadherin in oral squamous cell carcinoma (OSCC) development. This experimental study included an immunohistochemical analysis of Twist and E-cadherin proteins in paraffin-embedded specimens of oral leukoplakia (OL), OSCC, and normal oral mucosa. In addition, it was also performed a Western blot and double-immunofluorescence analysis of Twist and E-cadherin expression in OSCC cell lines. Significant differences in Twist and E-cadherin immunoexpression were observed between normal oral mucosa and OL, with an inverse relation since the earliest stages of oral dysplasia (r = -0,512; P < 0.001). Western blot and double-immunofluorescence analysis showed differences in Twist and E-cadherin expression among human oral keratinocytes and OSCC cell lines suggesting that downregulation of E-cadherin occurs in a dependent manner of Twist in OSCC. Our results showed a possible value of Twist and E-cadherin in the prediction of risk of oral epithelium malignant transformation.
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