Localization of BrdU-Induced Break Sites in Bovine Chromosomes
1983; University of Florence; Volume: 36; Issue: 3 Linguagem: Inglês
10.1080/00087114.1983.10797668
ISSN2165-5391
AutoresD. Di Berardino, L. Iannuzzi, G.P. Di Meo,
Tópico(s)Chromosomal and Genetic Variations
ResumoSUMMARYA map of BrdU-induced breaks in chromosomes of cattle has been achieved by using late BrdU incorporation for producing RBA bands in lymphocytes of a female calf affected by chromosome instability. This procedure gave a conspicuous rate of chromosome breaks which were carefully located on a diagrammatic representation of the RBA banded bovine karyotype arranged according to the Reading nomenclature. The sites of BrdU-induced breaks were found to be located at the junctions between R-positive (G-Q negative) and R-negative (G-Q positive) bands and, sometimes, between the centromere and the first subcentromeric band. The distribution of breaks among the chromosomes was found to be proportional to chromosome length. Highly sensitive BardU-break sites have been found in chromosomes 2, 12 and on the short arm of the early replicating X chromosome.
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