Portal de Periódicos da CAPES

Real-Time Metabolomics on Living Microorganisms Using Ambient Electrospray Ionization Flow-Probe

2013; American Chemical Society; Volume: 85; Issue: 15 Linguagem: Inglês

10.1021/ac401613x

1520-6882

Cheng‐Chih Hsu, Mariam ElNaggar, Yao Peng, Jinshu Fang, Laura M. Sanchez, Samantha J. Mascuch, Kirsten A. Møller, Emad K. Alazzeh, Jiří Pikula, Robert A. Quinn, Yi Zeng, Benjamin E. Wolfe, Rachel J. Dutton, Lena Gerwick, Lixin Zhang, Xueting Liu, Maria Månsson, Pieter C. Dorrestein,

Mass Spectrometry Techniques and Applications

Microorganisms such as bacteria and fungi produce a variety of specialized metabolites that are invaluable for agriculture, biological research, and drug discovery. However, the screening of microbial metabolic output is usually a time-intensive task. Here, we utilize a liquid microjunction surface sampling probe for electrospray ionization-mass spectrometry to extract and ionize metabolite mixtures directly from living microbial colonies grown on soft nutrient agar in Petri-dishes without any sample pretreatment. To demonstrate the robustness of the method, this technique was applied to observe the metabolic output of more than 30 microorganisms, including yeast, filamentous fungi, pathogens, and marine-derived bacteria, that were collected worldwide. Diverse natural products produced from different microbes, including Streptomyces coelicolor , Bacillus subtilis , and Pseudomonas aeruginosa are further characterized.