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Oral Aminoglycoside and Ofloxacin Therapy in the Prevention of Gram-Negative Sepsis after Irradiation

1991; Oxford University Press; Volume: 164; Issue: 5 Linguagem: Inglês

10.1093/infdis/164.5.917

1537-6613

Itzhak Brook, G. D. Ledney,

Bacterial Identification and Susceptibility Testing

To investigate whether oral gentamicin or ofloxacin therapy protects against gram-negative sepsis after irradiation, B6D2FI mice were exposed to 7.5 Gy of radiation from 60Co, infected with 107Pseudomonas aeruginosa or Klebsiella pneumoniae orally 3 days after irradiation, and treated with oral (15 mg/kg/day) or intramuscular (im; 7.5 mg/kg/day) gentamicin or oral (40 mg/kg/day) ofloxacin. For P. aeruginosa, gentamicin therapy was started orally 10 and 24 hand im 24 h after inoculation. For K. pneumoniae, gentamicin was started orally 24, 48, and 72 hand im 24 h after inoculation; ofloxacin was started 24 h after inoculation. Mice that received oral gentamicin early (10 h for P. aeruginosa, 24 h for K. pneumoniae), im gentamicin, or oral ofloxacin showed significantly (P < .05) reduced colonization, translocation, and mortality compared with mice that received oral gentamicin late. These data support the use of selective antimicrobial therapy to reduce colonization, translocation, and mortality from gram-negative bacteria in irradiated animals.