Portal de Periódicos da CAPES

A CONTROLLED ENVIRONMENT SYSTEM FOR STUDY OF MAMMALIAN CELL PHYSIOLOGY*

1966; Wiley; Volume: 139; Issue: 1 Linguagem: Inglês

10.1111/j.1749-6632.1966.tb41195.x

1749-6632

William F. McLimans, David T. Mount, Seymour Bogitch, Elizabeth J. Crouse, George F. Harris, George E. Moore,

Cell Image Analysis Techniques

Annals of the New York Academy of SciencesVolume 139, Issue 1 p. 190-213 A CONTROLLED ENVIRONMENT SYSTEM FOR STUDY OF MAMMALIAN CELL PHYSIOLOGY* William F. McLimans, William F. McLimans Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorDavid T. Mount, David T. Mount Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorSeymour Bogitch, Seymour Bogitch Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorElizabeth J. Crouse, Elizabeth J. Crouse Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorGeorge Harris, George Harris Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorGeorge E. Moore, George E. Moore Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this author William F. McLimans, William F. McLimans Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorDavid T. Mount, David T. Mount Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorSeymour Bogitch, Seymour Bogitch Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorElizabeth J. Crouse, Elizabeth J. Crouse Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorGeorge Harris, George Harris Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this authorGeorge E. Moore, George E. Moore Roswell Park Memorial Institute, New York State Department of Health, Buffalo, N. Y. and Spitz Laboratories, Yorklyn, Del.Search for more papers by this author First published: October 1966 https://doi.org/10.1111/j.1749-6632.1966.tb41195.xCitations: 24 * Contribution No. 1, Cell Physiology Facility: Investigations herein reported were conducted under Grant No. ES 30–02, United States Public Health Service, Bureau State Services. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 Bernard, C. 1857. Lecons sur les propriétés physiologiques des alterations pathologiques des liquides de l'organisme. Lecon d'ouverture. 1. Paris. Dec. 9, 1857. 2 Anderson, N. 1953. The mass isolation of whole cells from rat liver. Science 117: 627–628. 3 Kaltenbach, J. P. 1954. The preparation and utilization of whole cell suspensions obtained from solid mammalian tissues. Exp. Cell Res. 7: 568–571. 4 Laws, J. & L. Strickland. 1956. Metabolism of isolated liver cells. Nature. 178: 309–310. 5 Zimmerman, M., T. Delvin & M. Pruss. 1960. Anaerobic glycolysis of dispersed cell suspensions from normal and malignant tissues. Nature. 185: 315–316. 6 Kalant, H. & F. Young. 1957. Metabolic behavior of isolated liver and kidney cells. Nature. 179: 816–817. 7 Lata, G. & J. Reinerston. 1957. Cholesterol synthesis by isolated rat liver cells. Nature. 178: 47–48. 8 Westfall, B., E. V. Peppers & W. R. Earl. 1955. The change in concentration of certain constituents of the medium during growth of the strain HeLa cells. Amer. J. Hyg.. 61: 326–333. 9 Schwartz, B. 1960. A critical analysis of the closed system technique for lens culture. AMA Arch. of Ophthal. 63: 593–606. 10 Pious, D. A., R. N. Hamburger & S. E. Mills. 1963. Clonal analysis of primary human cultures. Fed. Proc.. 22(1324): 382. 11 Zaroff, L., G. Sato & S. E. Mills. 1961. Single cell platings from freshly isolated mammalian tissue. Exp. Cell Res.. 23: 565–575. 12 Levintow, L. & H. Eagle. 1961. Biochemistry of cultured mammalian cells. Ann. Rev. Biochem.. 30: 605–640. 13 Morgan, J. F. 1958. Tissue culture nutrition. Bact. Rev. 22: 20. 14 Eagle, H. 195859. Metabolic studies with normal and malignant human cells in culture. Harvey Lect.: 156–175. 15 Westfall, B. B., V. J. Evans, E. Peppers, N. M. Hawkins, J. C. Bryant, E. L. Schilling & W. R. Earle. 1958. Observations on the metabolic behavior of a clone of mouse liver cells grown in agitated fluid suspension. Cancer Res.. 18: 947–951. 16 Ehrmann, R. L. & G. O. Gey. 1956. The growth of cells on a transparent gel of reconstituted rat-tail collagen. J. Nat. Cancer Inst.. 16: 1375. 17 Hillis, W. D. & F. B. Bang. 1962. The cultivation of human embryonic liver cells. Exp. Cell Res.. 26: 9–36. 18 Dawson, P. S. S. 1963. A continuous-flow culture apparatus-The cyclone column unit. Canad. J. Microbiol. 9: 671–687. 19 Monod, J. & M. Cohn. 1952. La biosynthèse induite des enzymes. (Adaptation enzymatique). Advances Enzym.. 13: 67–119. 20 Novick, A. & L. Szilard. 1952. Experiments with the Chemostat on the Rates of Amino Acid Synthesis in Bacteria: 21–32. Princeton Univ. Press, Princeton , N. J. . 21 Herbert, D., R. Elsworth & R. C. Telling. 1956. The continuous culture of bacteria; A theoretical and experimental study. J. Gen. Microbiol.. 14: 601. 22 Pirt, S. J., E. J. Thackery & R. Harris-Smith. 1961. The influence of environment on antigen production by Pasteurella pestis studied by means of the continuous flow culture technique. J. Gen. Microbiol.. 25: 119–130. 23 Lacey, B. W. 1953. Three-dimensional patterns of antigenic modulation of Haemophilus pertussis, H. parapertussis and H. bronchisepticus. J. Gen. Microbiol. 8: iii. 24 Ogburn, C. A., T. N. Harris & J. Harris. 1958. Extracellular antigens in steady-state cultures of the hemolytic Streptococcus: Production of proteinase at low pH. J. Bact.. 76: 142. 25 Formal, S. B., L. S. Baron & W. Spilman. 1956. The virulence and immunogenicity of Salmonella typhos grown in continuous culture. J. Bact.. 72: 168–175. 26 Cooper, P. D., A. M. Burt & J. N. Wilson. 1958. Critical effect of oxygen tension on rate of growth of animal cells in continuous suspended culture. Nature. 182: 1508. 27 Graff, S. & K. S. McCarty. 1957. Sustained cell culture. Exp. Cell Res.. 13: 348. 28 McCoy, T. A., W. Whittle & E. Conway. 1961. A glass helix perfusion chamber for massive growth of cells in vitro. Proc. Soc. Exp. Biol. Med.. 109: 235–237. 29 Kruse, P. F., B. C. Myhr, J. E. Johnson & P. B. White. 1963. Perfusion system for replicate mammalian cell cultures in T-60 flasks. J. Nat. Cancer Inst.. 31: 109–123. 30 Bjorklund, B., V. Bjorklund & F. E. Paulsson. 1961. The cytostat, an apparatus for automatically controlled continuous propagation of suspended cells. Proc. Soc. Exp. Biol. Med.. 108: 385–390. 31 Thompson, K. W., M. M. Vicent, F. C. Jensen, R. T. Price & E. Schapiro. 1959. Production of hormones by human anterior pituitary cells in serial culture. Proc. Soc. Exp. Biol. Med.. 102: 403–408. 32 Cherry, W. R. & R. N. Hull. 1960. Studies on the growth of mammalian cells in agitated fluid media. Anat. Rec.. 124: 483. 33 Zwartouw, H. & J. Westwood. 1958. Factors affecting growth and glycolysis in tissue culture. Brit. J. Exp. Path.. 39: 529. 34 Stevens, K. M. 1965. Oxygen requirements for liver cells in vitro. Nature 206: 199. 35 Fenn, W. O., R. Gerschman, D. L. Gilbert, D. E. Terwilliger & F. V. Cathran. 1957. Mutagenic effects of high oxygen tensions on Escherichia coli. Proc. Nat. Acad. Sci.. 43: 1027–1032. 36 Liener, I. E. & D. L. Buchanan. 1957. The fixation of carbon dioxide by growing and non-growing yeast. J. Bact.. 61: 527–539. 37 Harris, J. O. 1954. The influence of carbon dioxide on oxygen uptake by resting cells of bacteria. J. Bact. 67: 476–479. 38 Dain, J. A., L. Neal & H. W. Seeley. 1956. The effect of carbon dioxide on polysaccharide production of Streptococcus bovis. J. Bact.. 72: 209–213. 39 Foster, J. W. 1949. Clinical Activities of the Fungi: 371. Academic Press Inc., New York , N. Y. . 40 Bauer, J. T. 1925. Effect of carbon dioxide on cells in tissue cultures. Bull. Hopkins Hosp. 37: 420–427. 41 Spratt, N. T. 1949. Carbon dioxide requirements of the early chick embryo. Anat. Rec. 105: 583. 42 Gwatkin, R. B. L. & L. Siminovitch. 1960. Multiplication of single mammalian cells in a nonbicarbonate medium. Proc. Soc. Exp. Biol. Med.. 103: 718. 43 Mottram, J. C. 1927. The role of carbon dioxide in the growth of normal and tumor cells. Lancet 213: 1232–1234. 44 Swim, H. E. & R. F. Parker. 1958. The role of carbon dioxide as an essential nutrient for six permanent strains of fibroblasts. J. Biophys. Biochem. Cyt.. 4(5): 525. 45 Loomis, W. F. 1957. Sexual differentiation in hydra. Control by carbon dioxide tension. Science 126: 735–739. 46 Chang, R. S. 1959. Participation of bicarbonate in RNA and protein synthesis as indicated by virus propagation in human cells. J. Exp. Med. 109: 229–238. 47 Brooks, S. C. & M. M. Brooks. 1941. The Permeability of Living Cells. Zehlendorf Verlag von Gebrüder Borntraeger, Berlin , W. Germany . 48 Danielli, J. F. 1937. The relations between surface pH, ion concentrations and interfacial tension. Proc. Roy. Soc. [Biol.] 122: 155–174. 49 Taylor, A. C. 1962. Responses of cells to pH changes in the medium. J. Cell. Biol. 15: 201–209. 50 Weiss, L. 1963. The pH value at the surface of Bacillus subtilis. J. Gen Microbiol. 32: 331–347. 51 Fischer, A. 1921. Growth of fibroblasts and hydrogen ion concentration of the medium. J. Exp. Med. 34: 447–454. 52 Lewis, M. R. & L. D. Felton. 1922. The hydrogen-ion concentration of tissue growth in vitro. Bull. Hopkins Hosp.. 33: 112–116. 53 Sankaran, G. 1933. Influence of hydrogen ion concentration of medium upon growth of fibroblasts in vitro. Indian J. Med. Res. 21: 189–196. 54 Gifford, G., H. Robertson & J. Syverton. 1956. Propagation in vitro of polioviruses VIII. Effect of pH on virus yield and cell metabolism. Proc. Soc. Exp. Biol. Med.. 93: 321–323. 55 Longsworth, L. G. & D. A. MacInnes. 1936. Bacterial growth with automatic pH control: A. An apparatus. B. Some tests on the acid production of Lactobacillus acidophilus. J. Bact.. 29: 595–607. 56 Kempe, L. L., H. O. Halvarson & E. L. Piret. 1950. Effect of continuously controlled pH on lactic acid fermentation. Ind. Eng. Chem.. 42: 1852–1855. 57 Mackenzie, C., J. Mackenzie & P. Beck. 1961. The effect of pH of growth, protein synthesis, and lipid-rich particles of cultured mammalian cells. J. Biophys. Biochem. Cyt.. 9: 141–156. 58 Vestergaard-Bogind, B. 1962. Automatic pH regulation of cell suspensions by a gasometric pH-stat. Scand. J. Clin. Lab. Invest. 14: 461–465. 59 Mount, D. T. & G. E. Moore. 1962. Continuous flow dialysis for environmental control of suspension cell culture. Tissue Culture Association Meeting, April, Washington, D. C.. 60 Giardinello, F. E., W. F. McLimans & G. W. Rake. 1958. The apparent toxicity of metallic materials of construction and antifoam agents for mammalian cell lines. Appl. Microbiol.. 6: 30. 61 Bergmeyer, H. V. 1963. Methods of Enzymatic Analysis. Academic Press, Inc. New York , N. Y. . 62 Hochella, N. J. & S. Weinhouse. 1964. Automated lactic acid determination in serum and tissue extracts. Technicon Symposium, April, New York City. 63 Friedland, I. M. & L. S. Dietrich. 1961. A rapid enzymic determination of L (+) – lactic acid. Anal. Biochem.. 2: 390–392. Citing Literature Volume139, Issue1Axenic Cultures and Defined MediaOctober 1966Pages 190-213 ReferencesRelatedInformation