EXPERIMENTAL SCARLET FEVER
1923; American Medical Association; Volume: 81; Issue: 14 Linguagem: Inglês
10.1001/jama.1923.02650140010004
ISSN1538-3598
Autores Tópico(s)Bacterial Identification and Susceptibility Testing
ResumoA nurse who was taking care of a patient with scarlet fever acquired the disease in a mild but typical form. Two days before the onset of symptoms, she noticed that she had a sore finger. On the second day of the disease, when the rash was intense, a few drops of pus were obtained from the lesion on her finger. Gram, Giemsa and Levaditi stains were made of direct smears of this pus. They contained many polymorphonuclear leukocytes and numerous single and paired gram-positive cocci; also an occasional gram-positive diphtheroid bacillus. Cultures of the pus were made on the surface of freshly prepared sheep's blood agar plates. Anaerobic cultures were made in tubes of ascites broth which contained pieces of fresh rabbit kidney, and on human blood agar slants. Smears and dark field preparations of the anaerobic cultures were examined at frequent intervals during two weeks' incubation. No organisms
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