High-performance liquid chromatography of membrane proteins
1987; Elsevier BV; Volume: 391; Linguagem: Inglês
10.1016/s0021-9673(01)94341-9
ISSN1873-3778
AutoresYoshio Kato, Takashi Kitamura, Koji Nakamura, Akane Mitsui, Yosuke Yamasaki, Tsutomu Hashimoto,
Tópico(s)Enzyme Structure and Function
ResumoVarious modes of high-performance liquid chromatography, gel filtration, ion-exchange chromatography, hydrophobic interaction chromatography, reversed-phase chromatography and metal chelate affinity chromatography, were investigated for the separation of membrane proteins. All were found applicable to membrane proteins, although the usefulness of each mode differed. For satisfactory results it was important to select appropriate elution conditions. The type and concentration of detergent was of special importance. The effects of other conditions, flow-rate, gradient steepness, type of buffer and salt, eluent pH, etc., were similar to those observed for soluble proteins.
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