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Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Utilization of two agroindustrial by-products for the production of a surfactant by Candida sphaerica UCP0995

2008; Elsevier BV; Volume: 43; Issue: 9 Linguagem: Inglês

10.1016/j.procbio.2008.04.013

1873-3298

Humberto Bezerra Souza Sobrinho, Raquel D. Rufino, Juliana M. Luna, Alexandra A. Salgueiro, Galba Maria de Campos‐Takaki, Lúcia Fernanda Cavalcanti da Costa Leite, Leonie Asfora Sarubbo,

Biofuel production and bioconversion

Medium constituents other than carbon sources affect the production of biosurfactants. In this work we describe a low cost medium for the production of a surfactant by the yeast Candida sphaerica. The medium was formulated only with distilled water supplemented with 5.0% ground-nut oil refinery residue plus 2.5% corn steep liquor as substrates. The isolated biosurfactant was formed with a yield of 4.5 gl−1. The biosurfactant showed high surface tension reducing activity (26 mN m−1), a small CMC value (0.08%), thermal and pH stability with respect to surface tension reducing activity and to emulsification activity against motor oil and a high level of salt tolerance. The biosurfactant was characterized as an anionic glycolipid consisting of 75% lipid and 25% carbohydrate. The potential application of the biosurfactant in oil recovery from sand was demonstrated by the percentile of oil removal (65%). The promising results obtained in this work are noteworthy for possible biosurfactant production from agricultural materials.