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Purification and characterization of bioactive peptides from skin extracts of Rana esculenta

1990; Elsevier BV; Volume: 1033; Issue: 3 Linguagem: Inglês

10.1016/0304-4165(90)90140-r

1872-8006

Maurizio Simmaco, Daniela De Biase, Cinzia Severini, M Aita, G. Falconieri Erspamer, Donatella Barra, Francesco Bossa,

Healthcare and Venom Research

The peptide fraction extracted by methanol from the skin of Rana esculenta, a species widely distributed in Western Europe, was investigated. The pharmacological activity found in the extract is attributable to the presence of authentic bradykinin, together with a shorter, partially active version of this molecule, des-Arg9-bradykinin. Also the bradykinin fragment 1–7 has been isolated, but it was inactive in our bioassay system. Moreover, a family of hydrophobic peptides has been purified and characterized, which appeared devoid of pharmacological activities when tested on smooth muscle preparations, but were provided with hemolytic activities.