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Polymorphism of interferon–gamma gene at position +874 and clinical characteristics of chronic hepatitis C

2006; Elsevier BV; Volume: 148; Issue: 3 Linguagem: Inglês

10.1016/j.trsl.2006.04.005

1931-5244

Chia‐Yen Dai, Wan‐Long Chuang, Ming‐Yen Hsieh, Li‐Po Lee, Nai‐Jen Hou, Shinn‐Cherng Chen, Zu‐Yau Lin, Ming‐Yuh Hsieh, Liang‐Yen Wang, Jun-Fa Tsai, Wen‐Yu Chang, Ming‐Lung Yu,

Cytokine Signaling Pathways and Interactions

A T-to-A polymorphic sequence at position +874 in the interferon (IFN)–gamma gene (+874 IFN–gamma) might be associated with disease susceptibilities. To investigate the influence of +874 IFN–gamma polymorphism on the hepatitis C virus (HCV) viral load and the severity of liver disease, the single nucleotide polymorphism (SNP) was determined in 302 histologically proved chronic hepatitis C (CHC) patients [M/F: 180/122, mean age: 48.8 ± 11.6 years, HCV genotype 1b: 147 (48.7%), liver cirrhosis: 29 (9.6%)] by using a polymerase chain reaction-sequence specific primers (PCR-SSP) approach. The distribution of genotypes for +874 IFN–gamma were T/T: 12 (4.0%), T/A: 71 (23.5%), and A/A: 219 (72.5%) and 27.5% (83/302) of patients' inherited T allele. The mean age of patients without A allele was significantly lower than other patients (41.7 ± 11.3 vs 49.2 ± 11.5 years, P = 0.028). Patients with the T allele of +874 IFN–gamma had a significantly higher rate of liver cirrhosis than patients with homozygote A allele (15.7% vs 7.3%, P = 0.028). By multivariate logistic regression analyses, T allele of +874 IFN–gamma and age were independent factors associated with cirrhosis (odds ratio/95% confidence interval: 2.519/1.128–5.622 and 1.065 /1.025–1.107, respectively). In conclusion, the authors' findings indicate that inheritance of +847 IFN–gamma polymorphism is associated with the cirrhosis in patients with CHC. A T-to-A polymorphic sequence at position +874 in the interferon (IFN)–gamma gene (+874 IFN–gamma) might be associated with disease susceptibilities. To investigate the influence of +874 IFN–gamma polymorphism on the hepatitis C virus (HCV) viral load and the severity of liver disease, the single nucleotide polymorphism (SNP) was determined in 302 histologically proved chronic hepatitis C (CHC) patients [M/F: 180/122, mean age: 48.8 ± 11.6 years, HCV genotype 1b: 147 (48.7%), liver cirrhosis: 29 (9.6%)] by using a polymerase chain reaction-sequence specific primers (PCR-SSP) approach. The distribution of genotypes for +874 IFN–gamma were T/T: 12 (4.0%), T/A: 71 (23.5%), and A/A: 219 (72.5%) and 27.5% (83/302) of patients' inherited T allele. The mean age of patients without A allele was significantly lower than other patients (41.7 ± 11.3 vs 49.2 ± 11.5 years, P = 0.028). Patients with the T allele of +874 IFN–gamma had a significantly higher rate of liver cirrhosis than patients with homozygote A allele (15.7% vs 7.3%, P = 0.028). By multivariate logistic regression analyses, T allele of +874 IFN–gamma and age were independent factors associated with cirrhosis (odds ratio/95% confidence interval: 2.519/1.128–5.622 and 1.065 /1.025–1.107, respectively). In conclusion, the authors' findings indicate that inheritance of +847 IFN–gamma polymorphism is associated with the cirrhosis in patients with CHC.