Portal de Periódicos da CAPES

Capillary electrophoresis of anions at high salt concentrations

1998; Wiley; Volume: 19; Issue: 12 Linguagem: Inglês

10.1002/elps.1150191216

1522-2683

Weiliang Ding, Michelle Thornton, James S. Fritz,

Electrowetting and Microfluidic Technologies

Abstract It is commonly thought that even a moderately high ionic concentration in the background electrolyte (BGE) would leaad to Joule heating and serious peak distortion. However, we obtained very satisfactory separations of both inorganic and organic anions in electrolyte solutions as high as 5 M sodium chloride using direct photometric detection. Samples containing a 0.5 M concentration of a salt can be analyzed directly by making the BGE concentration of the same salt even higher to obtain electrostacking. The temperature in the center of the capillary was calculated to be 49°C when the current is at its maximum of 280 μA. The effect of various salts on electrophoretic and electroosmotic mobility is discussed. Several examples are given of capillary electrophoresis under high‐salt conditions.