A new method for the physical and genetic mapping of large plasmids: application to the localisation of the virulence determinants on the 90 kb plasmid of Salmonella typhimurium
1987; Elsevier BV; Volume: 3; Issue: 2 Linguagem: Inglês
10.1016/0882-4010(87)90069-6
ISSN1096-1208
AutoresThomas Michiels, M.Y. Popoff, Serge Durviaux, C Coynault, Guy R. Cornelis,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoA new method based on transposon-promoted deletions was used to generate a set of deletions in the 90 kb virulence plasmid of Salmonella typhimurium. The analysis of 16 deletion mutants allowed: (1) construction of the restriction map of the plasmid for HindIII, BamHI and BglII; (2) localisation of the plasmid region involved in virulence; (3) identification of two functional replicons on the plasmid.
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