Comparison of agar and microcrystal cellulose as gelling agents forin vitro culture ofNicotiana tabacum stem expiants
1993; Institute of Experimental Botany of the Czech Academy of Sciences; Volume: 35; Issue: 2 Linguagem: Inglês
10.1007/bf02925942
ISSN1573-8264
AutoresN. Gorinova, A Atanasov, K. S. Alexandrova, R. Velkova, A. Kasachka,
Tópico(s)Light effects on plants
ResumoInvestigation was made on a use of microcrystal cellulose as a new and inexpensive gelling agent instead of agar. Microcrystal cellulose in concentration 20 % forms a suitable structure of nutrient medium for in vitro cultivation. The higher humidity in the culture container with microcrystal cellulose causes partial vitrification of Nicotiana tabacum L. plants, cv. Zlatna arda. It is proved by reduced chlorophyll content, changes in protein synthesis and strongly reduced isoenzyme spectrum of peroxidase.
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