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Knockdown of Pokemon Protein Expression Inhibits Hepatocellular Carcinoma Cell Proliferation by Suppression of AKT Activity

2013; Cognizant Communication Corporation; Volume: 20; Issue: 8 Linguagem: Inglês

10.3727/096504013x13657689383012

1555-3906

Xiaosan Zhu, Yichen Dai, Zhangxin Chen, Jun-Pei Xie, Wei Zeng, Yuanyuan Lin,

Acute Myeloid Leukemia Research

Overexpression of Pokemon, which is an erythroid myeloid ontogenic factor protein, occurs in different cancers, including hepatocellular carcinoma (HCC). Pokemon is also reported to have an oncogenic activity in various human cancers. This study investigated the effect of Pokemon knockdown on the regulation of HCC growth. POK shRNA suppressed the expression of Pokemon protein in HepG2 cells compared to the negative control vector-transfected HCC cells. Pokemon knockdown also reduced HCC cell viability and enhanced cisplatin-induced apoptosis in HCC cells. AKT activation and the expression of various cell cycle-related genes were inhibited following Pokemon knockdown. These data demonstrate that Pokemon may play a role in HCC progression, suggesting that inhibition of Pokemon expression using Pokemon shRNA should be further evaluated as a novel target for the control of HCC.