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Identification and characterization of glycosyltransferases involved in the biosynthesis of soyasaponin I in Glycine max

2010; Wiley; Volume: 584; Issue: 11 Linguagem: Inglês

10.1016/j.febslet.2010.03.037

1873-3468

Masaaki Shibuya, Kazuya Nishimura, Nao Yasuyama, Yutaka Ebizuka,

Ginseng Biological Effects and Applications

Triterpene saponins are a diverse group of compounds with a structure consisting of a triterpene aglycone and sugars. Identification of the sugar‐transferase involved in triterpene saponin biosynthesis is difficult due to the structural complexity of triterpene saponin. Two glycosyltransferases from Glycine max , designated as GmSGT2 and GmSGT3, were identified and characterized. In vitro analysis revealed that GmSGT2 transfers a galactosyl group from UDP‐galactose to soyasapogenol B monoglucuronide, and that GmSGT3 transfers a rhamnosyl group from UDP‐rhamnose to soyasaponin III. These results suggest that soyasaponin I is biosynthesized from soyasapogenol B by successive sugar transfer reactions.