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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Canine visceral leishmaniosis: A comparative analysis of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos and the IFI-leishmaniose-visceral-canina-Bio-Manguinhos kits

2006; Elsevier BV; Volume: 137; Issue: 1-2 Linguagem: Inglês

10.1016/j.vetpar.2005.12.020

ISSN

1873-2550

Autores

Rodrigo Araújo Lira, Milena Cavalcanti, M Nakazawa, Antonio Ferreira, Edmilson Silva, Frederico G. C. Abath, Lêucio Câmara Alves, Wayner Vieira de Souza, Yara M. Gomes,

Tópico(s)

Leptospirosis research and findings

Resumo

This study evaluated the performance of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos (EIE-LVC) kit and to compare it with that of the IFI-leishmaniose-visceral-canina-Bio-Manguinhos (IFI-LVC) kit. Four groups of dogs were studied: group 1 (G1), dogs with clinical signs indicative of CVL and testing positive for the parasite (n = 25); group 2 (G2), dogs with only a presumed diagnosis of CVL (n = 62); group 3 (G3), dogs that had never lived in an area where CVL is endemic and never received a blood transfusion (n = 16); group 4 (G4), dogs carrying other parasites: such as babesiosis (n = 4), ehrlichiosis (n = 6) and demodicosis (n = 1). G1 and G3 were used for the calculation of sensitivity and specificity, respectively. The EIE-LVC showed a sensitivity of 72% (IC 95%: 50.4–87.1%) and a specificity of 87.5% (IC 95%: 60.4–97.8%). The value of the kappa index was 0.975 (CI 95%: 0.926–1.024), which represents an excellent fit. For IFI-LVC, the sensitivity was 68.0% (CI 95%: 46.4–84.3%) and the specificity 87.5% (CI 95%: 60.4–97.8%). When the tests were conducted in parallel, sensitivity was 92.0% (CI 95%: 72.5–98.6%) and specificity 75.0% (CI 95%: 47.4–91.7%). However, when conducted consecutively, the tests showed a sensitivity of 48.0% (CI 95%: 28.3–68.2%) and a specificity of 100.0% (CI 95%: 75.9–99.4%). The analysis of clinically suspected dogs using IFI-LVC and EIE-LVC kits in parallel, revealed that 26/62 animals were positive. Cross-reaction was observed in a dog with demodicosis. These results lead to the following conclusions: (1) the performance of the EIE-LVC kit is not statistically different from the IFI-LVC and (2) the kits must be used in parallel if higher sensitivity is required, reducing the number of false-negative results.

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