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Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Clinically Important Yeast Species

2010; American Society for Microbiology; Volume: 48; Issue: 10 Linguagem: Inglês

10.1128/jcm.00687-09

1098-660X

Lindsay G. Stevenson, Steven K. Drake, Yvonne R. Shea, Adrian M. Zelazny, Patrick R. Murray,

Genomics and Phylogenetic Studies

ABSTRACT We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of yeast species. Using Bruker Daltonics MALDI BioTyper software, we created a spectral database library with m/z ratios of 2,000 to 20,000 Da for 109 type and reference strains of yeast (44 species in 8 genera). The database was tested for accuracy by use of 194 clinical isolates (23 species in 6 genera). A total of 192 (99.0%) of the clinical isolates were identified accurately by MALDI-TOF MS. The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparation time.