Fast enzymatic preparation of l-DOPA from tyrosine and molecular oxygen: a potential method for preparing [15O]l-DOPA
1990; Pergamon Press; Volume: 41; Issue: 9 Linguagem: Inglês
10.1016/0883-2889(90)90066-p
ISSN1878-1128
AutoresJacques Maddaluno, Kym F. Faull,
Tópico(s)Advanced Proteomics Techniques and Applications
ResumoA fast, simple and inexpensive enzymatic preparation of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) from molecular oxygen and tyrosine using mushroom tyrosinase is described. The theoretical incubation time for production of [15O]l-DOPA with maximal specific activity from [15O]O2 can be calculated to be about 3 min. In practice, using a specially-designed glass reaction chamber to facilitate the incorporation of gaseous molecular oxygen into l-DOPA with zero lag-time, a 3 min reaction with 1% oxygen in nitrogen results in the formation of approx. 3.9 μmol of l-DOPA, representing conversion of about 14% of the tyrosine substrate. Given access to a supply of [15O]O2, the method should be applicable to the preparation of [15O]l-DOPA for use as a PET tracer.
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