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Anti-HEV antibodies in domestic animal species and rodents from Spain using a genotype 3-based ELISA

2009; Elsevier BV; Volume: 137; Issue: 1-2 Linguagem: Inglês

10.1016/j.vetmic.2009.01.006

1873-2542

Bibiana Peralta, Maribel Casas, Nilsa de Deus, Marga Martı́n, A. Ortuño, Eva Pérez-Martín, Sonia Pina-Pedrero, Enric Mateu,

Viral gastroenteritis research and epidemiology

A truncated ORF2 capsid HEV antigen derived from a genotype 3 strain was developed in insect cells and insect larvae, and compared with the Sar55 antigen and a commercial ELISA. The antigen expressed in insect cells showed a better correlation with Sar55 (kappa value (k) = 0.84) than the insect larvae antigen (k = 0.69), and a better reproducibility as indicated by the intra and interplate variation coefficients. Commercial ELISA designed for human diagnosis but adapted to animal use using specific secondary antibodies demonstrated to have a very low sensitivity. The insect cell expressed antigen was used to develop an ELISA to detect anti-HEV-IgG in serum samples of different domestic animal and rodents. Seropositivity in the studied animal populations was 71.4% for pigs, 0.60% for goats, 1.92% for sheep, and 11.11% for cats. None of the 1170 cattle samples or 166 rodent samples analyzed was positive.