Portal de Periódicos da CAPES

Physiological Proteomics of the Uncultured Endosymbiont of Riftia pachyptila

2007; American Association for the Advancement of Science; Volume: 315; Issue: 5809 Linguagem: Inglês

10.1126/science.1132913

1095-9203

Stephanie Markert, C. Arndt, Horst Felbeck, Dörte Becher, Stefan M. Sievert, Michael Hügler, Dirk Albrecht, Julie Robidart, Shellie R. Bench, Robert A. Feldman, Michael Hecker, Thomas Schweder,

Protist diversity and phylogeny

The bacterial endosymbiont of the deep-sea tube worm Riftia pachyptila has never been successfully cultivated outside its host. In the absence of cultivation data, we have taken a proteomic approach based on the metagenome sequence to study the metabolism of this peculiar microorganism in detail. As one result, we found that three major sulfide oxidation proteins constitute ∼12% of the total cytosolic proteome, which highlights the essential role of these enzymes for the symbiont's energy metabolism. Unexpectedly, the symbiont uses the reductive tricarboxylic acid cycle in addition to the previously identified Calvin cycle for CO 2 fixation.