Large-scale isolation and purification of geniposide from the fruit of Gardenia jasminoides Ellis by high-speed counter-current chromatography
2005; Elsevier BV; Volume: 1100; Issue: 1 Linguagem: Inglês
10.1016/j.chroma.2005.09.026
ISSN1873-3778
AutoresTingting Zhou, Guorong Fan, Zhanying Hong, Yifeng Chai, Yutian Wu,
Tópico(s)Biological Activity of Diterpenoids and Biflavonoids
ResumoHigh-speed counter-current chromatography (HSCCC) was applied to the isolation and purification of geniposide from Gardenia jasminoides Ellis. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of ethyl acetate–n-butanol–water (2:1:3, v/v/v). According to the above solvent system, preparative HSCCC was successfully performed with the optimal solvent system composed of ethyl acetate–n-butanol–water (2:1.5:3, v/v/v) yielding 389 mg of geniposide at over 98% purity from 1 g of the partially purified extract with 38.9% recovery in a one-step separation.
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