Early DNA-binding polypeptides of Epstein-Barr virus
1981; Elsevier BV; Volume: 113; Issue: 1 Linguagem: Inglês
10.1016/0042-6822(81)90155-0
ISSN1096-0341
AutoresJ. Roubal, Bengt Kallin, János Luka, George Klein,
Tópico(s)T-cell and Retrovirus Studies
ResumoThe kinetics of Epstein-Barr virus (EBV)-specific protein synthesis has been studied after induction of P3HR-1 cells with n-butyrate. The majority of the EBV polypeptides became detectable on SDS-polyacrylamide gel electrophoresis between 15 and 18 hr after addition of the drug. Overall viral protein synthesis was at maximum after about 24 hr. Some polypeptides appeared significantly earlier (152K, 55K, 51K) while others were synthesized during a limited period of the cycle only (65K). At least four of the polypeptides detected (152K, 134K, 55K, 51K) bound to DNA cellulose columns. The EBV specificity of the polypeptides was confirmed by immunoprecipitation and by two-dimensional gel analysis. The 152K polypeptide had a pI around 8 whereas the other polypeptides varied between basic and acidic pI. The DNA binding proteins isolated by DNA cellulose chromatography represent a subclass of the virus-specific early proteins that can be immunoprecipitated from the total cell extract.
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