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The CentO satellite confers translational and rotational phasing on cenH3 nucleosomes in rice centromeres

2013; National Academy of Sciences; Volume: 110; Issue: 50 Linguagem: Inglês

10.1073/pnas.1319548110

1091-6490

Tao Zhang, Paul B. Talbert, Wenli Zhang, Yufeng Wu, Zhenglin Yang, Jorja G. Henikoff, Steven Henikoff, Jiming Jiang,

Plant Disease Resistance and Genetics

Significance Centromeres are sites on chromosomes that mediate attachment to microtubules for chromosome segregation and often comprise tandemly repeated “satellite” sequences. The function of these repeats is unclear because centromeres can be formed on single-copy DNA by the presence of nucleosomes containing a centromere-specific variant of histone H3 (cenH3). Rice has centromeres composed of both the 155-bp CentO satellite repeat and single-copy non- CentO sequences. This study shows that rice cenH3 nucleosomes are regularly spaced with 155-bp periodicity on CentO repeats, but not on non- CentO sequences. CentO repeats have an ∼10-bp periodicity in dinucleotide pattern and in nuclease cleavage that suggests that CentO has evolved to minimize its bending energy on cenH3 nucleosomes and that centromeric satellites evolve for stabilization of cenH3 nucleosomes.