Stabilization and particle morphology of prune dwarf virus
1978; Elsevier BV; Volume: 91; Issue: 2 Linguagem: Inglês
10.1016/0042-6822(78)90389-6
ISSN1096-0341
AutoresEdward L. Halk, Robert W. Fulton,
Tópico(s)Animal Virus Infections Studies
ResumoBelow ionic concentrations of about 0.02 M the virions of prune dwarf virus (PDV) lost infectivity rapidly and sedimented heterogenously in sucrose density gradients. The virus was stable in EDTA or salt solution of 0.03 to 0.3 M. Infectivity was lost quickly in solutions of divalent cations. When stabilized, PDV sedimented in sucrose density gradients as five closely spaced zones of about 75, 81, 85, 98 and 113 S. Infectivity was associated with the 98 and 113 S zones. Formaldehyde-fixed virus equilibrated as a single zone at a density of 1.330 in CsCl gradients. Only one zone appeared upon free boundary electrophoresis, but 5 or 6 zone appeared after electrophoresis in 2.8% polyacrylamide gels. The virus contained 14% RNA. Stabilized virus from the upper zone of sucrose density gradient tubes was essentially isometric, with a diameter of 19–20 nm. An increasing proportion of particles from more rapidly sedimenting zones were oval to bacilliform with widths of 17–19 nm and lengths up to 73 nm.
Referência(s)