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Production of endo-1,4-β-glucanase and xylanase with nylon-web immobilized and free Trichoderma reesei

1996; Elsevier BV; Volume: 18; Issue: 7 Linguagem: Inglês

10.1016/0141-0229(95)00156-5

1879-0909

Reetta Haapala, Elke Parkkinen, Pirkko Suominen, S. Linko,

Enzyme Production and Characterization

On all media studied, the endo-1,4-β-glucanase and xylanase activities obtained with the nylon-web immobilized Trichoderma reesei were higher than the activities with the free mycelium. The nitrogen source had a marked effect on endoglucanase and xylanase production on lactose, cellulose, and l-sorbose-based medium with immobilized T. reesei. The highest enzyme activities with this medium, 880 nkat ml−1 for endoglucanase and 8,020 nkat ml−1 for xylanase, were obtained in the presence of proteose peptone and yeast extract. Urea alone was an insufficient nitrogen source for enzyme production. A lactose-containing industrial by-product-based medium offered a good alternative for endoglucanase and xylanase production. In this case, the maximum endoglucanase activity was 940 nkat ml−1, xylanase activity was 4,410 nkat ml−1, and filter paper activity was 1.9 FPU ml−1. On synthetic cellulose-based medium, the activities were 840 nkat ml−1, 4.790 nkat ml−1, and 3.0 FPU ml−1, respectively. The nylon-web immobilized biocatalyst could be used for two successive batch cultures with only a little decrease in enzyme production observed.