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Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells

1990; Wiley; Volume: 260; Issue: 1 Linguagem: Inglês

10.1016/0014-5793(90)80083-u

1873-3468

Daisuke Yamauchi, Takao Minamikawa,

Phytase and its Applications

We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata . The sequence covers the whole transcribed region as well as the 5'‐ and 3'‐untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G‐50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.