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Degradation of Photosystem II reaction center D1-protein induced by UVB radiation in isolated thylakoids. Identification and characterization of C- and N-terminal breakdown products

1994; Elsevier BV; Volume: 1184; Issue: 1 Linguagem: Inglês

10.1016/0005-2728(94)90156-2

1879-2650

Giulia Friso, Cornelia Spetea, Giorgio M. Giacometti, Imre Vass, Roberto Barbato,

Algal biology and biofuel production

The effects of UVB radiation on the stability of Photosystem II reaction center D1-protein in isolated thylakoids are investigated. A new C-terminal degradation product has been identified and characterized, produced by cleavage in the second transmembrane segment. Immunological evidence for the presence of N-terminal fragments corresponding to the remaining part of D1-protein is also provided. The appearance of these fragments is not affected by lowering the temperature from 22°C to 4°C, changing the pH from 6 to 8, adding soybean trypsin inhibitor or excluding oxygen from the thylakoid suspension.