Isolation and Characterization of Monoclonal Antibodies Monospecific for Bovine α-Casein and β-Casein
1991; Elsevier BV; Volume: 74; Issue: 3 Linguagem: Inglês
10.3168/jds.s0022-0302(91)78228-3
ISSN1529-9066
AutoresKonrad M. Kuzmanoff, John W. Andresen, Craig W. Beattie,
Tópico(s)Protein purification and stability
ResumoTwo monoclonal antibodies monospecific for bovine %lcasein, which recognize three genetic variants of ql-casein, have been isolated and their binding properties characterized.Antibodies 57-115 and 57-310 recognize different antigenic determinants on the o&l protein with affinity constants of 1.63 x 10" and 2.13 x 10" W1, respectively.Five monoclonal antibodies, 58-409, monospecific for bovine p-casein with affinity constants >lo9 M-l, which recognize a similar epitope(s) also were isolated.All seven antibodies are of isotype IgGl and recognize both the denatured and undenatured forms of their antigen, making them suitable for qualitative and quantitative radioimmunoassay.
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