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Human and canine gene homologs of porcine brain natriuretic peptide

1989; Elsevier BV; Volume: 165; Issue: 2 Linguagem: Inglês

10.1016/s0006-291x(89)80015-4

1090-2104

Jeffrey J. Seilhamer, Ann Arfsten, Judith Miller, Penny Lundquist, Robert M. Scarborough, John Lewicki, J G Porter,

Advanced biosensing and bioanalysis techniques

A cDNA clone encoding porcine brain natriuretic peptide (BNP) precursor was used to probe a canine genomic DNA library for homologous sequences. A unique clone was obtained (D1) which encoded a peptide homologous to porcine BNP. A 4.0 kB Hind III fragment of D1 containing the entire BNP precursor coding region was hybridized against blots of EcoRI digested human genomic DNA. A 7.0 kB hybridizing band was observed, which was subsequently subcloned and sequenced. This human clone, H1, also contained a sequence homologous to porcine BNP precursor. Transcription of the human BNP gene was confirmed by detection of BNP-specific sequences amplified from human atrial RNA by polymerase chain reaction. These results suggest that both BNP and ANP are present and function in a wide variety of mammalian species.