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Simultaneous detection of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

2010; Elsevier BV; Volume: 22; Issue: 2 Linguagem: Inglês

10.1016/j.foodcont.2010.05.021

1873-7129

Chai Fung Pui, Woan Chwen Wong, Lay Ching Chai, Elexson Nillian, Farinazleen Mohamad Ghazali, Yoke Kqueen Cheah, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi, Son Radu,

Identification and Quantification in Food

This study aimed to determine the prevalence and quantity of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia. Analysis was carried out using the most probable number (MPN) – multiplex polymerase chain reaction (PCR) method. The prevalence of Salmonella spp., Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23.3%, 7.6% and 3.8%, respectively with estimated quantity varying from 0 to 19 MPN/g. This study urged the authority to look into the biosafety of sliced fruits in Malaysia.