The isolation of a toxin from the dahlia sea anemone, Tealia felina L.
1986; Elsevier BV; Volume: 24; Issue: 2 Linguagem: Inglês
10.1016/0041-0101(86)90113-3
ISSN1879-3150
AutoresR.C. Elliott, R.S. Konya, K. Vickneshwara,
Tópico(s)Neurobiology and Insect Physiology Research
ResumoGel chromatography with Dyematrex blue B gel and ion exchange chromatography with a methylcellulose cation exchange column have been used to isolate a toxin present in partially purified saline extracts of the dahlia sea anemone Tealia felina. Toxin activity was assessed by its histaminolytic activity. A ten-fold increase in activity per mg protein was achieved in these two steps. Final purification on a Sephacryl column yielded an apparently pure protein with a molecular weight of 7800. The amino acid composition of this compound was determined.
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