Produção Nacional
Revisado por pares
Improved detection of incipient vascular changes by a biotechnological platform combining post mortem MRI in situ with neuropathology
2009; Elsevier BV; Volume: 283; Issue: 1-2 Linguagem: Inglês
10.1016/j.jns.2009.02.327
ISSN1878-5883
AutoresLea T. Grinberg, Edson Amaro, Alexandre Valotta da Silva, Rafael Emídio da Silva, João Ricardo Sato, Denis Dionizio dos Santos, Silmara de Paula Pacheco, Renata Eloah de Lucena Ferretti‐Rebustini, Renata Elaine Paraízo Leite, Carlos Augusto Pasqualucci, Stefan Teipel, Wilhelm Flatz, Helmut Heinsen,
Tópico(s)Medical Image Segmentation Techniques
ResumoAbstract The histopathological counterpart of white matter hyperintensities is a matter of debate. Methodological and ethical limitations have prevented this question to be elucidated. We want to introduce a protocol applying state-of-the-art methods in order to solve fundamental questions regarding the neuroimaging–neuropathological uncertainties comprising the most common white matter hyperintensities [WMHs] seen in aging. By this protocol, the correlation between signal features in in situ , post mortem MRI-derived methods, including DTI and MTR and quantitative and qualitative histopathology can be investigated. We are mainly interested in determining the precise neuroanatomical substrate of incipient WMHs. A major issue in this protocol is the exact co-registration of small lesion in a tridimensional coordinate system that compensates tissue deformations after histological processing. The protocol is based on four principles: post mortem MRI in situ performed in a short post mortem interval, minimal brain deformation during processing, thick serial histological sections and computer-assisted 3D reconstruction of the histological sections. This protocol will greatly facilitate a systematic study of the location, pathogenesis, clinical impact, prognosis and prevention of WMHs.
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