Identification of Luciferyl Adenylate and Luciferyl Coenzyme A Synthesized by Firefly Luciferase
2003; Wiley; Volume: 5; Issue: 1 Linguagem: Inglês
10.1002/cbic.200300735
ISSN1439-7633
AutoresHugo Fraga, Joaquim C. G. Esteves da Silva, Rui Fontes,
Tópico(s)Nicotinic Acetylcholine Receptors Study
ResumoAbstract The firefly luciferase reaction intermediate luciferyl adenylate was detected by RP‐HPLC analysis when the luciferase reaction was performed under a nitrogen atmosphere. Although this compound is always specified as an intermediate in the light‐production reaction, this is the first report of its identification by HPLC in a luciferase assay medium. Under a low‐oxygen atmosphere, luciferase can catalyze the synthesis of luciferyl coenzyme A from luciferin, ATP, and coenzyme A, but in air dehydroluciferyl coenzyme A was produced. The luciferase‐catalyzed synthesis of these coenzyme A derivatives may be a consequence of the postulated recent evolutionary origin of firefly luciferases from an ancestral acyl‐coenzyme A synthetase.
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