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Mitophagy confers resistance to siderophore-mediated killing by Pseudomonas aeruginosa

2015; National Academy of Sciences; Volume: 112; Issue: 6 Linguagem: Inglês

10.1073/pnas.1424954112

1091-6490

Natalia V. Kirienko, Frederick M. Ausubel, Gary Ruvkun,

Vibrio bacteria research studies

Significance Pathogens express virulence factors to support their growth and reproduction while hosts activate various immune processes to promote pathogen clearance and minimize damage. In this study, we establish a new role for pyoverdin, an iron-binding siderophore produced by the bacterium Pseudomonas aeruginosa . In addition to promoting growth by acquiring iron, pyoverdin serves as a secreted bacterial toxin that disrupts mitochondria and iron homeostasis in Caenorhabditis elegans . We show that exposure to pyoverdin triggers mitochondrial damage and subsequent mitophagy (lysosomal degradation of damaged mitochondria). Importantly, mitophagy confers a protective effect against exposure to either pyoverdin or to a synthetic iron chelator, demonstrating a function for mitophagy in innate immunity. Finally, we show that iron chelation causes mitophagy in mammalian cells.