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Basic Fibroblast Growth Factor Regulates Matrix Metalloproteinases Production and In Vitro Invasiveness in Human Bladder Cancer Cell Lines

1997; Lippincott Williams & Wilkins; Volume: 157; Issue: 6 Linguagem: Inglês

10.1016/s0022-5347(01)64779-7

1527-3792

Hideaki Miyake, Koji Yoshimura, Isao Hara, Hiroshi Eto, Soichi Arakawa, Sadao Kamidono,

Epigenetics and DNA Methylation

No AccessJournal of UrologyInvestigative Urology1 Jun 1997Basic Fibroblast Growth Factor Regulates Matrix Metalloproteinases Production and In Vitro Invasiveness in Human Bladder Cancer Cell Lines Hideaki Miyake, Koji Yoshimura, Isao Hara, Hiroshi Eto, Soichi Arakawa, and Sadao Kamidono Hideaki MiyakeHideaki Miyake , Koji YoshimuraKoji Yoshimura , Isao HaraIsao Hara , Hiroshi EtoHiroshi Eto , Soichi ArakawaSoichi Arakawa , and Sadao KamidonoSadao Kamidono View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)64779-7AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: This study was designed to investigate the effect of endogenous basic fibroblast growth factor (FGF-2) on matrix metalloproteinases (MMPs) production and in vitro invasive potential of human bladder cancer cell lines. Materials and Methods: The human bladder cancer cell lines, HT1376 and KoTCC-1, were used in this study. The mRNA for FGF receptor has been shown to be expressed in both cell lines; the mRNA for FGF-2 is expressed in only KoTCC-1. The effects of FGF-2 expression on HT1376 by gene transfection and those of FGF-2 antisense oligonucleotides treatment on KoTCC-1 were analyzed by zymography and in vitro tumor cell invasion assay. Results: The introduction of human FGF-2 gene into HT1376 cells markedly enhanced both the MMP-2 and MMP-9 production, and the in vitro invasive potential was also increased. In contrast, the exposure of KoTCC-1 cells to FGF-2 specific antisense oligonucleotides decreased the MMP-2 production and in vitro invasive potential, but the exposure to FGF-2 sense oligonucleotides did not. Conclusions: These findings suggest that FGF-2 plays an important role in the invasive process of human bladder cancer in part through the regulation of MMPs production. References 1 : Current management of invasive and metastatic transitional cell carcinoma of the bladder. J. 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Google Scholar From the Department of Urology, Kobe University School of Medicine, Kobe, Japan.© 1997 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byYoussef R, von Rundstedt F, Kapur P, Mosbah A, Abol-Enein H, Ghoneim M and Lotan Y (2014) Utility of Biomarkers in the Prediction of Oncologic Outcome after Radical Cystectomy for Squamous Cell CarcinomaJournal of Urology, VOL. 193, NO. 2, (451-456), Online publication date: 1-Feb-2015.MIYAKE H, ETO H, HARA I, SO A, LI D and GLEAVE M (2018) SYNERGISTIC ANTITUMOR ACTIVITY BY COMBINED TREATMENT WITH GEMCITABINE AND ANTISENSE OLIGODEOXYNUCLEOTIDE TARGETING CLUSTERIN GENE IN AN INTRAVESICAL ADMINISTRATION MODEL AGAINST HUMAN BLADDER CANCER KOTCC-1 CELLSJournal of Urology, VOL. 171, NO. 6 Part 1, (2477-2481), Online publication date: 1-Jun-2004.MUNRO N and KNOWLES M (2018) Fibroblast Growth Factors and Their Receptors in Transitional Cell CarcinomaJournal of Urology, VOL. 169, NO. 2, (675-682), Online publication date: 1-Feb-2003.HARA I, MIYAKE H, HARA S, ARAKAWA S and KAMIDONO S (2018) SIGNIFICANCE OF MATRIX METALLOPROTEINASES AND TISSUE INHIBITORS OF METALLOPROTEINASE EXPRESSION IN THE RECURRENCE OF SUPERFICIAL TRANSITIONAL CELL CARCINOMA OF THE BLADDERJournal of Urology, VOL. 165, NO. 5, (1769-1772), Online publication date: 1-May-2001.MENDELSOHN J and DINNEY C (2018) THE WILLET F. WHITMORE, JR., LECTURESHIP: BLOCKADE OF EPIDERMAL GROWTH FACTOR RECEPTORS AS ANTICANCER THERAPYJournal of Urology, VOL. 165, NO. 4, (1152-1157), Online publication date: 1-Apr-2001.MIYAKE H, HARA I, YAMANAKA K, ARAKAWA S and KAMIDONO S (2018) SYNERGISTIC ENHANCEMENT OF RESISTANCE TO CISPLATIN IN HUMAN BLADDER CANCER CELLS BY OVEREXPRESSION OF MUTANT-TYPE p53 AND Bcl-2Journal of Urology, VOL. 162, NO. 6, (2176-2181), Online publication date: 1-Dec-1999. Volume 157 Issue 6 June 1997 Page: 2351-2355 Advertisement Copyright & Permissions© 1997 by American Urological Association, Inc.Metrics Author Information Hideaki Miyake More articles by this author Koji Yoshimura More articles by this author Isao Hara More articles by this author Hiroshi Eto More articles by this author Soichi Arakawa More articles by this author Sadao Kamidono More articles by this author Expand All Advertisement PDF downloadLoading ...