Femtosecond cellular transfection using a nondiffracting light beam
2007; American Institute of Physics; Volume: 91; Issue: 5 Linguagem: Inglês
10.1063/1.2766835
ISSN1520-8842
AutoresX. Tsampoula, V. Garcés‐Chávez, M. Comrie, D. J. Stevenson, B. Agate, C. T. A. Brown, Frank J. Gunn‐Moore, Kishan Dholakia,
Tópico(s)Microfluidic and Bio-sensing Technologies
ResumoThe ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam.
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