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Analysis of rat serum transcortin-steroid hormone association by column chromatography

1977; Elsevier BV; Volume: 180; Issue: 1 Linguagem: Inglês

10.1016/0003-9861(77)90017-0

1096-0384

M.K. Agarwal,

Estrogen and related hormone effects

Transcortin-bound gluco- and mineralocorticoids were fractionated on a number of chromatographic systems. Contrary to earlier suggestions of a homogenous unit by competition binding and Scatchard analysis, a polymorphic nature of the globulin was evident with corticosterone on Sephadex A-50 columns (components in 0.4 and 0.6 m KCl) and with synthetic steroids (triamcinolone acetonide, dexamethasone) or natural mineralocorticoids (aldosterone, 18-hydroxy-deoxy-corticosterone) on diethylaminoethyl cellulose-52 gels (species in 0.001 and 0.06 m phosphate). Besides the major component of molecular weight 55,000, a heavier shoulder in the 67,000 molecular weight region was obtained with cortisol, dexamethasone, triamcinolone, and aldosterone from Sephadex G-200 columns, on which binding was reduced in the presence of high salt (0.4 m KCl). Triamcinolone- and dexamethasone-bound components eluted at lower salt concentrations from the DE-52 column than natural steroid-corticosteroid-binding globulin complexes. The various features of serum carrier binding are discussed in terms of steroid-receptor association in hormone-specific target tissues.