Production and purification of alternansucrase, a glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355, for the synthesis of oligoalternans
1993; Elsevier BV; Volume: 15; Issue: 1 Linguagem: Inglês
10.1016/0141-0229(93)90120-q
ISSN1879-0909
AutoresAgustín López‐Munguía, Vincent Pelenc, M. Remaud, Jacques Biton, J.-M. Michel, Christine Lang, F. Paul, Pierre Monsan,
Tópico(s)Plant Gene Expression Analysis
ResumoAlternansucrase, a glucosyltransferase from Leuconostoc mesenteroides NRRL B-1355, was produced by fed-batch cultures and purified by two-phase partition with polyethylene glycol. The contaminating dextransucrase activity was eliminated by heat treatment. Some properties of the enzyme were examined, in particular its transferase activity in the presence of maltose. A general procedure for oligoalternan production and purification is defined.
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