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DNA adducts in carp exposed to artificial diesel-2 oil slicks

1992; Elsevier BV; Volume: 228; Issue: 1 Linguagem: Inglês

10.1016/0926-6917(92)90011-z

1878-6782

Branko Kurelec, Achal Garg, Sanja Krča, Smiljana Britvić, Davor Lučıć, Ramesh C. Gupta,

Pharmaceutical and Antibiotic Environmental Impacts

In attempts to mimic field exposure, oil slicks prepared from diesel-2 oil/water emulsions were poured onto the surface of water in tanks prepared fresh every day and liver DNA adducts were analyzed by 32P-postlabeling in carp free-swimming in these tanks. ‘Clusters’ of lipophilic DNA adducts were detected, with five major and numerus minor adducts. Essentially a similar adduct pattern was found in the liver DNA of carp exposed to crude oil-polluted water. Diesel-2 adduct induction was observed slowly with a steady increase to > 3000 amol/μg DNA at day 12. After this time fish were transferred to clean water. Adduct levels continued to increase through day 17 (≈ 10,000 amol/μg DNA) despite the cessation of exposure, but a 30% and 80% decline was evident at day 22 and day 27, respectively. All major adducts were distinct from the known benzo[a]pyrene diolepoxide-dG. These results indicate that diesel-2 oil can cause extensive DNA damage in carp in vivo and the damage accumulates proportionately with time of exposure.