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Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network

2004; Nature Portfolio; Volume: 6; Issue: 2 Linguagem: Inglês

10.1038/ncb1090

1476-4679

Charles Yeaman, Inmaculada Ayala, Jessica R. Wright, Frédéric Bard, Carine Bossard, Agnes Ang, Yusuke Maeda, Thomas Seufferlein, Ira Mellman, W. James Nelson, Vivek Malhotra,

Pancreatic function and diabetes

Protein kinase D (PKD) binds to diacylglycerol (DAG) in the trans-Golgi network (TGN) and is activated by trimeric G-protein subunits βγ. This complex then regulates the formation of transport carriers in the TGN that traffic to the plasma membrane in non-polarized cells. Here we report specificity of different PKD isoforms in regulating protein trafficking from the TGN. Kinase-inactive forms of PKD1, PKD2 and PKD3 localize to the TGN in polarized and non-polarized cells. PKD activity is required only for the transport of proteins containing basolateral sorting information, and seems to be cargo specific.