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Stimulation of GCMa and syncytin via cAMP mediated PKA signaling in human trophoblastic cells under normoxic and hypoxic conditions

2005; Wiley; Volume: 579; Issue: 18 Linguagem: Inglês

10.1016/j.febslet.2005.06.029

1873-3468

Ina Knerr, Steffen Schubert, Christina Wich, Kerstin Amann, Thomas Aigner, Tina Vogler, Ronny Jung, Jörg Dötsch, Wolfgang Rascher, Said Hashemolhosseini,

Neonatal Health and Biochemistry

Glial cells missing a (GCMa) belongs to a new transcription factor family. Syncytin was shown to be a target gene of GCMa. Here, we demonstrate that the protein kinase A (PKA) pathway acts upstream of GCMa. After transient transfection of BeWo cells with PKA, GCMa transcriptional activity and both GCMa and syncytin transcripts were upregulated. This increase was accompanied by further cellular differentiation. Using normoxic or hypoxic conditions to mimic pathophysiological settings known to diminish trophoblast differentiation, we found that gene repressive effects of oxygen deficiency were compensated by the induction of the PKA pathway. We propose that GCMa-driven syncytin expression is the key mechanism for syncytiotrophoblast formation.