Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

The PGE2/IL-10 Axis Determines Susceptibility of B-1 Cell-Derived Phagocytes (B-1CDP) to Leishmania major Infection

2015; Public Library of Science; Volume: 10; Issue: 5 Linguagem: Inglês

10.1371/journal.pone.0124888

1932-6203

Angélica Arcanjo, Isabel Ferreira LaRocque-de-Freitas, Juliana Dutra B. Rocha, Daniel Zamith‐Miranda, Ana Caroline Costa-da-Silva, Marise P. Nunes, Fabio P. Mesquita-Santos, Alexandre Morrot, Alessandra A. Filardy, Mário Mariano, Christianne Bandeira‐Melo, George A. DosReis, Débora Decoté‐Ricardo, Célio Geraldo Freire-de-Lima,

Trypanosoma species research and implications

B-1 cells can be differentiated from B-2 cells because they are predominantly located in the peritoneal and pleural cavities and have distinct phenotypic patterns and activation properties. A mononuclear phagocyte derived from B-1 cells (B-1CDP) has been described. As the B-1CDP cells migrate to inflammatory/infectious sites and exhibit phagocytic capacity, the microbicidal ability of these cells was investigated using the Leishmania major infection model in vitro. The data obtained in this study demonstrate that B-1CDP cells are more susceptible to infection than peritoneal macrophages, since B-1CDP cells have a higher number of intracellular amastigotes forms and consequently release a larger number of promastigotes. Exacerbated infection by L. major required lipid bodies/PGE2 and IL-10 by B-1CDP cells. Both infection and the production of IL-10 were decreased when PGE2 production was blocked by NSAIDs. The involvement of IL-10 in this mechanism was confirmed, since B-1CDP cells from IL-10 KO mice are more competent to control L. major infection than cells from wild type mice. These findings further characterize the B-1CDP cells as an important mononuclear phagocyte that plays a previously unrecognized role in host responses to L. major infection, most likely via PGE2-driven production of IL-10.