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High-yield purification of small, single-stranded DNA

1981; Elsevier BV; Volume: 115; Issue: 1 Linguagem: Inglês

10.1016/0003-2697(81)90546-7

1096-0309

Robert A. Sclafani, James A. Wechsler,

RNA Interference and Gene Delivery

Several simple methods that aid in the high-yield purification of small, single-stranded DNA are described. The methods rely initially on the silicone coating of laboratory ware with a particular brand of commercial silicone coating. The quantitative precipitation of DNA with cetyltrimethylammonium bromide and the fractionation of the DNA by molecular-sieve chromatography allowed purification based on length of the polynucleotide strands. For purification of density-labeled DNA, Cs2SO4 isopycnic centrifugation and precipitation by cetyltrimethylammonium bromide gave good purification of the DNA of interest even at sizes as small as ∼200 nucleotides.