Isolation and structure of the nucleocapsid of HVJ
1968; Elsevier BV; Volume: 35; Issue: 3 Linguagem: Inglês
10.1016/0042-6822(68)90223-7
ISSN1096-0341
Autores Tópico(s)Animal Virus Infections Studies
ResumoA method for isolation of the nucleocapsids of HVJ from virions was developed: HVJ virions suspended in Na2CO3NaHCO3 buffer (0.02 M, 1:1) were disintegrated with Emasol 1130 (a Tween 20-like surfactant) and banded by CsCl equilibrium centrifugation. The nucleocapsids of HVJ formed a band at a buoyant density of 1.31, and most of the envelopes and partially disintegrated virions formed a top layer. The nucleocapsids thus obtained retain their full length and have a sedimentation constant S20,w, of about 250S, but no detectable infectivity for eggs. The nucleocapsids contain about 3.7% RNA, but no detectable phospholipid or DNA. The nucleocapsids mainly consist of one antigenic component, named S, in gel diffusion. Treatment of the nucleocapsids with trypsin or RNase has no significant effect on its main morphology or on sedimentation or antigenicity of the S component. The nucleocapsid is a single helix, probably right-handed and with 15 subunits per turn. Some of the characteristics of the disintegration of HVJ virions by the present alkali-Emasol treatment are also described.
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