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Precise large deletions by the PCR-based overlap extension method

1995; Springer Science+Business Media; Volume: 4; Issue: 1 Linguagem: Inglês

10.1007/bf02907467

1559-0305

Savithra D. Senanayake, David A. Brian,

Bacteriophages and microbial interactions

The authors describe an efficient method for generating large deletions (>200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.