Electron Microscope Study of Epidermophyton Floccosum11From the Medical Service, Radioisotope Service, and General Medical Research Service, General Medical and Surgical Hospital, Veterans Administration Center, the Division of Dermatology, Department of Medicine, and the Department of Radiology, University of California Medical Center, Los Angeles, Calif.
1958; Elsevier BV; Volume: 31; Issue: 1 Linguagem: Inglês
10.1038/jid.1958.77
ISSN1523-1747
AutoresEdward L. Laden, John O. Erickson,
Tópico(s)Antifungal resistance and susceptibility
ResumoDetails of bacterial cell structure have been elucidated to some extent through electron microscopy of ultrathin sections. Less has been reported on the application of this relatively new technique to the problem of fungal architecture (1-4) and virtually nothing has been published on thin section studies of dermatophytes, so well recognized clinically as the causative agents in numerous human cutaneous infections. This paper describes exploratory experiments on thin section electronmicroscopy of Epidermophyton floccosum. METHODS Approximately 1 mm. cubes were cut from the periphery of 14 day colonies of E. floccosum grown on solid Sabouraud's medium at room temperature. Some of these were fixed in buffered 1% osmium tetroxide (5) and others in buffered 0.6% potassium permanganate. Still others were fixed in buffered 1 % chromate, 1 % lead acetate, and 1.5% phosphotungstate. Specimens after fixation for 2-16 hours were dehydrated through the usual alcohol series and embedded in n-butyl methacrylate. Sectioning was performed on a Porter-Blum microtome with glass knives (6). The sections were mounted on carbon films and examined in an RCA EMU microscope fitted with a 25 or 50 ju objective aperture.
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